Introduction: Infants perceive pain more than children and adults. Vaccination is one of the most common painful procedures in infants, so various methods have been proposed to assess the pain related vaccination and decrease the pain.. The main object of this study is to compare the effects of seed therapy and breastfeeding on pain related vaccination in infants. Methods and materials: In a randomized clinical trial, 96 infants 6-12 months who were referred to health care center for HB , and DTP or MMR vaccination were allocated randomly. After parents consents they were assigned to one of the breastfeeding, seed therapy and control groups.Their pain were assessed by neonatal infant pain scale(NIPS) immediately after injection. Data were analyzed by descriptive analytic methods. T). Results: The results showed that the means of pain score were significantly different between three groups (P< 0/001). Post hoc LSD test showed that the differences between the breastfeeding and control groups (P <0/001) , breastfeeding and seed therapy groups (P = 0/002) and also control and seed therapy groups (P = 0/02) were statistically significant. Conclusion: Results showed that both methods of seed therapy and breastfeeding were effective for decreasing pain related vaccination in infants 6 and 12 months. In addition the effect of breastfeeding was significantly more than seed therapy. Key words: breastfeeding, pain, seed therapy,Vaccination

Keywords: Vaccination, pain, breastfeeding, seed therapy