TY - JOUR JF - CMJA JO - cmja VL - 9 IS - 4 PY - 2020 Y1 - 2020/3/01 TI - Response of Liver Tissue Bax and Bcl-2 Gene Expression to Aerobic Training with L-Carnitine Supplementation in Rats Toxicated by Boldenone TT - پاسخ بیان ژن Bcl-2 و Bax بافت کبد به یک دوره تمرین هوازی به همراه مکمل ال‌کارنیتین در موش‌های مسموم‌شده با بولدنون N2 - Objective: This study aimed to compare the response of liver tissue BAX and BCL-2 gene expression to aerobic training with L-carnitine supplementation in rats intoxicated by Boldenone. Methods: In this experimental study, 30 male Wistar rats, aged 12 weeks (weight 195±7.94 g) were randomly divided into five groups: Control, no-treatment, boldenone (5 mg/kg), L-carnitine (100 mg/kg) and aerobic training- L-carnitine. The moderate endurance intensity training program (50%-55% of maximal oxygen consumption) performed for 6 weeks and 5 times a week. Injection once a week in the quadriceps and hamstring was conducted in-depth. After anesthesia, an autopsy was performed, and the liver Isolated. The hepatic apoptosis gene expression in the samples was measured by Real-Time PCR. Data were analyzed by 1-way ANOVA and post hoc Scheffe at the significant level P<0.05. Results: Significant difference was observed between the mean expression of BAX and BCL-2 in the liver tissue of male Wistar rats in different groups (P=0.001). The BAX gene expression of the liver tissue in L-carnitine -aerobic training and L-carnitine groups was significantly lower than the Boldenon group (P=0.001). Also, The BCL-2 gene expression in L-carnitine- aerobic exercise and L-carnitine groups was significantly higher than the Boldenon group (P=0.001). Conclusion: According to the findings of this study, supplementation of L-carnitine with regular aerobic training can have a protective effect against apoptosis induced by anabolic-androgenic steroids. SP - 3890 EP - 3901 AU - Ahmadi, Mozhgan AU - Abbassi Daloii, Asieh AU - Salehi Kiasari, Samira AD - Department of Physical Education and Sport Science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. KW - Aerobic training KW - Boldenone KW - L-carnitine KW - Apoptosis KW - Wistar rats UR - http://cmja.arakmu.ac.ir/article-1-658-en.html DO - 10.32598/cmja.9.3.896.1 ER -