Volume 15, Issue 3 (12-2025)
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Askari B, Falahati Shahabodini M, Farajtabar Behrestaq S. Effect of Eight Weeks of Aerobic Training Combined with Resveratrol Consumption on the Expression of Telomeric Repeat Binding Factors 1 and 2 and Telomerase Reverse Transcriptase in Liver Tissue in Rats with Non-Alcoholic Fatty Liver Disease. cmja 2025; 15 (3)
URL: http://cmja.arakmu.ac.ir/article-1-1050-en.html
URL: http://cmja.arakmu.ac.ir/article-1-1050-en.html
1- Department of Physical Education and Sport Sciences, QaS.C., Islamic Azad University, Qaemshahr, Iran , b.askari.1359@iau.ac.ir
2- Department of Physical Education and Sport Sciences, QaS.C., Islamic Azad University, Qaemshahr, Iran
2- Department of Physical Education and Sport Sciences, QaS.C., Islamic Azad University, Qaemshahr, Iran
Abstract: (16 Views)
Introduction: Age-related diseases (such as liver disease) are associated with relatively short telomeres, and severe telomere shortening is a prerequisite for reduced lifespan. The present study aimed to investigate the effect of eight weeks of aerobic training combined with resveratrol on the expression of telomere repeat splicing factor 1 and 2, and telomerase reverse transcriptase, in the liver tissue of rats with non-alcoholic fatty liver disease (NAFLD).
Methods: To conduct the present experimental study, 35 eight-week-old male Wistar rats were randomly divided into two groups: non-alcoholic fatty liver and control. Rats in the control group were fed a standard diet for 6 weeks, while rats in the experimental group were fed a high-fat diet for 6 weeks to induce NAFLD. The rats in the patient group were again divided into four experimental subgroups: NAFLD, NAFLD-training, Non-alcoholic fatty liver-resveratrol, and NAFLD-training-resveratrol. The rats in the exercise group performed an eight-week (5 days a week) aerobic interval training program. Mice in the supplement group were injected intraperitoneally with 20 mg/kg of resveratrol daily for eight weeks. Forty-eight hours after the completion of the research protocol, gene expression levels in liver tissue were measured using Real-time PCR. Finally, to compare the mean changes of the research variables, one-way analysis of variance and Tukey's post hoc test were used at a significance level of P<0.05.
Results: The results showed a significant increase in the rate of changes in telomeric repeat binding factor 1 and telomerase reverse transcriptase expression in NAFLD groups (P=0.0001) and a significant decrease in the rate of changes in telomeric repeat binding factor 2 expression in NAFLD groups (P=0.0001) compared to the control group. Moreover, a significant decrease in the rate of changes in telomeric repeat binding factor 1 expression was observed in the NAFLDT (P=0.042) and NAFLDTRSV (P=0.0001) groups compared to NAFLD. A significant increase in the rate of changes in telomeric repeat binding factor 2 expression was observed in the NAFLDT (P=0.030), NAFLDRSV (P=0.020), and NAFLDTRSV (P=0.0001) groups compared to the NAFLD group. Furthermore, a significant decrease in telomerase reverse transcriptase expression was observed in the NAFLDT (P=0.040), NAFLDRSV (P=0.049), and NAFLDTRSV (P=0.0001) groups compared to the NAFLD group.
Conclusion: Considering the role of shelterins and telomerases in cellular function, it seems that changing the levels of these indicators following physical activity and the use of resveratrol supplementation can partially prevent the occurrence of many liver diseases caused by NAFLD.
Methods: To conduct the present experimental study, 35 eight-week-old male Wistar rats were randomly divided into two groups: non-alcoholic fatty liver and control. Rats in the control group were fed a standard diet for 6 weeks, while rats in the experimental group were fed a high-fat diet for 6 weeks to induce NAFLD. The rats in the patient group were again divided into four experimental subgroups: NAFLD, NAFLD-training, Non-alcoholic fatty liver-resveratrol, and NAFLD-training-resveratrol. The rats in the exercise group performed an eight-week (5 days a week) aerobic interval training program. Mice in the supplement group were injected intraperitoneally with 20 mg/kg of resveratrol daily for eight weeks. Forty-eight hours after the completion of the research protocol, gene expression levels in liver tissue were measured using Real-time PCR. Finally, to compare the mean changes of the research variables, one-way analysis of variance and Tukey's post hoc test were used at a significance level of P<0.05.
Results: The results showed a significant increase in the rate of changes in telomeric repeat binding factor 1 and telomerase reverse transcriptase expression in NAFLD groups (P=0.0001) and a significant decrease in the rate of changes in telomeric repeat binding factor 2 expression in NAFLD groups (P=0.0001) compared to the control group. Moreover, a significant decrease in the rate of changes in telomeric repeat binding factor 1 expression was observed in the NAFLDT (P=0.042) and NAFLDTRSV (P=0.0001) groups compared to NAFLD. A significant increase in the rate of changes in telomeric repeat binding factor 2 expression was observed in the NAFLDT (P=0.030), NAFLDRSV (P=0.020), and NAFLDTRSV (P=0.0001) groups compared to the NAFLD group. Furthermore, a significant decrease in telomerase reverse transcriptase expression was observed in the NAFLDT (P=0.040), NAFLDRSV (P=0.049), and NAFLDTRSV (P=0.0001) groups compared to the NAFLD group.
Conclusion: Considering the role of shelterins and telomerases in cellular function, it seems that changing the levels of these indicators following physical activity and the use of resveratrol supplementation can partially prevent the occurrence of many liver diseases caused by NAFLD.
Type of Study: Research |
Subject:
Physiology
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