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Volume 12, Issue 1 (In Press 2022)                   cmja 2022, 12(1): 0-0 | Back to browse issues page

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Rasouli fooshazdeh A, Abedi B, Matinhomaee H, Shokati P. The effect of aerobic exercise and Tribulus terrestris extract on some indicators of oxidative stress and apoptosis in lung tissue of male rats poisoning with hydrogen peroxide. cmja. 2022; 12 (1)
URL: http://cmja.arakmu.ac.ir/article-1-874-en.html
1- Islamic Azad University
2- Islamic Azad University , abedi@iaumahallat.ac.ir
Abstract:   (196 Views)
Introduction: Oxidative stress and apoptosis due to hydrogen peroxide poisoning have a major effect on the physiological and pathophysiological mechanisms of the lung. The aim of this study was to evaluate the effect of aerobic exercise and Tribulus terrestris extract on some indicators of oxidative stress and lung tissue apoptosis in poisoned male Wistar rats.
Materials and Methods: In an experimental design, 49 male rats aged 10 to 12 weeks were randomly divided into 7 groups: Control, Poisoned, Poisoning+ Exercise, Poisoning+ Tribulus terrestris 1 (5 mg/kg), Poisoning+ Tribulus terrestris 2 (10 mg/kg), Poisoning+ Exercise+ Tribulus terrestris 1, Poisoning+ Exercise+ Tribulus terrestris 2. The aerobic exercise program consisted of eight weeks (5 days a week) running on a treadmill at a speed of 20 meters per minute for 60 minutes per session. Alcoholic extract after administration at doses of 5 and 10 mg/kg was administered to the target animals by gavage for eight weeks. Serum levels of oxidative stress indices were measured with special kits and the expression of apoptotic indices of lung tissue was measured by immunohistochemistry. The research hypotheses were analyzed using two-way analysis of variance and LSD post hoc test at a significance level of 0.05.
Results: Eight weeks of aerobic exercise significantly increased adenosine triphosphate (ATP) (F=19.238, P=0.002, ƞ=0.468) and significantly decreased cytochrome C concentration (F=68.970, P=0.001, ƞ=0.697) and pulmonary oxidant-prooxidant balance (PAB) (F=12.025, P=0.001, ƞ=0.464), while having a significant effect on values. Had no malondialdehyde (MDA) (F=1.067, P=0.169, ƞ=0.044). Receiving two doses of Tribulus terrestris extract significantly decreased PAB index (F=10.118, P=0.001, ƞ=0.403) and receiving 10 mg of it increased ATP (F=35.040, P=0.001, ƞ=0.701) and significantly decreased cytochrome C (F=13.730, P=0.004, ƞ=0.417) and MDA (F=5.824, P=0.002, ƞ=0.372) concentrations. The expression of Bax protein (F=54.247, P=0.001, ƞ=0.804), (F=51.201, P=0.002, ƞ=0.754)   and caspase 3 (F=49.118, P=0.003, ƞ=0.504), (F=49.001, P=0.004, ƞ=0.498)   after receiving 5 and 10 mg of Tribulus terrestris extract and eight weeks of aerobic exercise significantly decreased and BCl-2 expression (F=63.014, P=0.001, ƞ=0.815), (F=50.112, P=0.003, ƞ=0.706) increased. The most changes in the evaluated indices were observed when combining aerobic exercise with 10 mg of Tribulus terrestris extract.
Conclusion: Apparently, 8 weeks of aerobic exercise is a good way to reduce the effects of oxidative stress and apoptosis due to hydrogen peroxide poisoning. However, the effects of Tribulus terrestris extract may be dose-dependent in some cases. Although the interaction of aerobic exercise with Tribulus terrestris has better results in controlling oxidative stress enzymes and pulmonary apoptotic factors, for better results, longer training periods and higher doses should be used.

     
Type of Study: Research | Subject: Physiology

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