1 2228-5091 Arak university of Medical Sciences 99 Effect of acupressure on severity of pain in women with multiple sclerosis (MS). Bastani Farideh b Sobhani Marzieh c Bozorgnejad Mehri d Shamsikhani Soheila e Haghani Hamid f b Tehran University of Medical Sciences, c Tehran University of Medical Sciences, d Tehran University of Medical Sciences, e Arak University of Medical Sciences, f Tehran University of Medical Sciences, 1 6 2012 2 1 75 84 25 07 2012 09 08 2014 Background: Multiple sclerosis is the most common chronic demyelization of central nervous system disease. The prevalence of MS is much more common in women than men. Pain is a symptom in MS that affects the patients’ quality of life reduces the ability of patients to do perform their daily activities. Therefore there are the pharmacological and non-pharmacological methods to control the symptom. Among non-pharmacological methods, acupressure, is a low-cost and noninvasive procedure that can be used to control symptoms of the disease. The aim of this study was to investigate the effect of acupressure on severity of pain in women with MS. Methods: In this single blind randomized clinical controlled trial, one hundred women were recruited from Tehran MS association. Subjects were divided equally into two groups of experimental and placebo, by random allocation method. Visual analog scale was used to evaluate the severity of pain. The intervention for experimental group was acupressure in ST36, SP6, LI4 points for 3 minutes bilaterally. In addition, the participants were instructed to perform acupressure in same points twice daily for two weeks. Subjects in placebo group were trained to touch the same points. The severity of pain was measured in both groups immediately, two and four weeks after intervention. The data were analysis by SPSS version 17. Results: There were no significant differences in severity of pain (p=0.16) at baseline in two groups. There was significant reduction of severity of pain in experimental group compared to placebo group immediately, two and four weeks after intervention respectively (p=0.01, p=0.01, p=0.02). Conclusion: According to the study results, acupressure on ST36, SP6, LI4 reduced symptom of pain in women with MS. Therefore the intervention is safe, simple, non-invasive and low-cost, it is recommended, to nurses to practice and educate the procedure to the client in relation to self-care and health promotion. Key word: Multiple Sclerosis, Pain, Acupressure.
86 Apoptotic Effect of milk thistle extract on human breast cancer MCF-7 cell Bashash Davood Davood g Safa Majid h Shahbazi Atefeh i Mohammadian Mozhdeh j Shah-Mohammad Narges k g Shahid Beheshti University of Medical Sciences, TehranIran,Iran. h Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran. i Islamic azad university, Science and Research Campus, Kurdistan branch, Sanandaj ,Iran.. j Tabriz University of Medical Sciences k Tehran University of Medical Sciences. 1 6 2012 2 1 85 95 19 06 2012 05 07 2014 Background: Given the prevalence of breast cancer in Iran, the increased incidence of this malignancy during the past two decades, the high mortality rate of patients, and according to oral consumption, low cost and easy access to the public extracts of milk thistle, in this study we aimed to investigate the effectiveness of this herb on MCF-7 breast cancer cells. Material and Methods: In order to investigate the effect of silibinin in breast cancer, MCF-7 cells were cultured in the presence of different concentrations of the drug. MTT assay, Immunoblotting, and quantitative real-time PCR were used to assess the effect of the drug on cell viability and the expression levels of mRNA and protein of Bax and Bcl-2 genes. Results: Evaluation of cell survival using the MTT assay showed that silibinin reduced the viability of MCF-7 cells in a time and dose dependent manner. The results of this study showed that silibinin significantly increased levels of Bax mRNA expression, while the amount of Bcl-2 gene expression was not affected. As expected according to the results of real-time PCR, expression level of Bax protein was also increased significantly, whereas the Bcl-2 protein level was not changed. Discussion: Overall, the results of this study confirm the efficacy of this herb against breast cancer. Therfore, the milk thistle as a natural herb supplement with low cost and public access, could be effective in breast cancer therapy. Key words: silibinin, breast cancer, MCF-7 cells, Bax, Bcl-2 85 Effect of massage therapy on somatic symptoms of postmenopausal women Taavoni simin l Dorsare Fatemeh m Julaei Sudabeh n Haghani Hamid o l Tehran University of Medical Sciences. Member of Research Institute for Islamic & Complementary Medicine (RICM, TUMS), Tehran, Islamic Republic of Iran m Tehran University of Medical Sciences. n Tehran University of Medical Sciences. o Statistics Department, School of Management and Medical Information & Health Sciences, Tehran University of Medical Sciences, Tehran, Iran 1 6 2012 2 1 96 103 19 06 2012 05 07 2014 Back ground: women have gone through many changes both physically and mentally during menopause, which may have negative impact on their quality of life. Therefore finding methods to manage this period of life is important. Many women are interested to use complementary and alternative medicines for relief from their menopausal complications. The purpose of this study was to compare the effect Massage therapy on somatic symptoms. Method: This randomized controlled trial study was carried out on 60 menopausal women who attended to menopausal clinic. They were randomly divided in 2 groups as intervention groups and control. Each subject in the massage group received two sessions massage training and six massage therapy sessions, twice a week with plane oil, and no treatment was provided to subjects in the control group. The outcome measures in this study were somatic symptoms, as obtained through the Menopause Rating Scale. Results: There was no significant difference in somatic symptoms before intervention between groups. A statistically significant difference was found between the participants’ pre-application and post-application in hot flush, sleep problem and muscle problem scores in intervention groups (p<0.05), whereas the heart problem score did not differed significantly. Conclusion: This study showed that massage therapy were effective in reducing some somatic symptoms. Therefore midwives can learn it and teach it to menopausal women in order to manage the menopausal symptoms. Evaluating the effect of massage therapy on other symptoms of menopause is recommended. Key words: Massage therapy, Somatic symptoms, Hot flush, Sleep problems. 89 Effect of BL32 and GB21 acupressure on labor pain in the first stage of labor seyyedzadeh aghdam nafiseh p vakilian katayoon masoomeh dawood abadi p MSc, Academic member of Nursing Midwifery School of Arak University of MedicalSciences, Arak, Iran Faculty of Nursing &Midwifery. Arak University of Medical Science. Arak. Iran. . Arak University of Medical Science. Arak. Iran. 1 6 2012 2 1 104 110 19 06 2012 05 07 2014 Introduction: delivery is a natural and painful phenomen .acupressure is one of the alternative methods for pain managment . . because of the side effects of drug using.the purpose of this study is to determine effect of acupressure for relief of labor pain in the first stage of labor is assesed. Methods :a single blind clinical trial designed for assessment of effect of acupressure on labor pain .sampling method was non randomized and available samples but samples divided randomize to two groups including65 persons as case group and 65 person as control group . acupressure conducted with cupping method . case group received BL32 and GB21 acupressure and control group received placebo p6 point acupressure for one minute in the start of every contraction in the first stage of labor . pain severity was measured by use of VAS tool and demographic characters was measured by questionnaire . softwaire of spss and Ttest was used for analysis . Results :findings indicated that mean of severity of pain difference between two groups before intervention was insignificantly with (p=0/019)but after intervention in the case group was more than control group significantly with (p=0/039). Conclusions : :results of this study showed that acupressure with above named method non effective on labor pain in examined women. Key words ::-acupressure –BL32– -GB21-labor pain 92 The Study of the Olive Oil Effect on the Second Degree Burn in the Experimental Mice farahani Molud Rahzani Kobra mojtabaei Mohadeseh Maleki Rad Aliakbar Sofian Masome Master of Nursing,Instructor, Faculty of Nursing and Midwifery,Arak University of Medical Sciences. Arak, Iran Faculty of Nursing and Midwifery,Arak University of Medical Sciences.Arak, Iran Shahid Beheshti University of Medical Sciences. Tehran, Iran. Assistant Professor,PhD of Physiology, Departement of Biology , Iran. Associate Professor,M.D., Faculty of Medicine,Arak University of Medical Sciences,Tuberculosis and pediatric infectious research center. Arak, Iran. 1 6 2012 2 1 111 118 19 06 2012 05 07 2014 Authors: Kobra Rahzani, Aliakbar Maleki Rad, Seyed Mahmod Tabatabaee,Masome Sofian, Shazand Payame Noor university Introduction: Burn is one of the most common injury that causes many people to be affected each year and to recover it, different methods are used. Olive oil is used as one of the treatment methods a long time ago. The aims: Therefore a study is done on the survey of olive oil effect in the recovery of the wounds which are caused by the burn. The way of study: This study is an experimental study on 30 male adult westar mice After anesthesia, a second degree burn in 2.5 cm Diameter was created in the side bodies of the mice by the boil water in 95 for three seconds. Then the mice were occasionally divided into three groups. For treatment in the first group olive oil, and in the second group sulfadiazine silver cream %1 and in the third one normal salin were used. In each group the time of the recover was recorded in the special list. The results were analysized with the use of the descriptive analysis such as mean, deviation standard and analytic analysis such as Mann Withney U. Results and Conclusion: The mean and deviation standard (mean SD) time of burn recovery in the first, the second and the third groups were in this manner 13.63 0.52- 11.25 0.89 and 17.75 0.76 day. The use of the Mann Withney U shows a significant different between the A and B groups in compartment with the C group (P<0.5). The use of olive oil caused acceleration in the burn injury recovery but in comparison Sulfadiazine Silver Cream a long time is needed for the burn injury recovery. Key Words: Second degree burn, olive oil, Sulfadiazine Silver Cream. 90 Comparison of the effect of oxytocin IM injection and nipple stimulation on bleeding and length of third stage of delivery Narenji Fereshteh Bahmani Zahra Mirzaeie Razieh Academic member, Dept, of Maternity Nursing Arak University Healthy Network of Varamin, Shahidbehshti University. Shohada Hospital, Ilam University. 1 6 2012 2 1 119 125 19 06 2012 05 07 2014 Introduction : Third stage of delivery is one of important stage of delivery and is still one of the most common causes of mothers death in third world countries . Methods : It is a clinical trial study to compare the effect of injection of oxytocin to mothers intramuscularly , nipple stimulation and physiologic manangment of third stage of delivery on length and bleeding range of this stage on 150 pregnant women from first to fourth pregnancy , referring to labor room of taleghaani hospital . The purpose of present study is research which were placed accidentally into three groups . in the first group immediately after delivery and cutting the umbilical cord , 10 units of oxytocin was injected into the mothers muscle , in the second group , right after delivery and cutting umbilical cord and take the baby in mother breast and the last group manage physiologically and did not receive any medicines . Then length and range of bleeding of the third stage of delivery in each group was studied and analyzed statistically .. results : Findings indicated that the average age of pregnant woman who were participated in this study was about (0/406)+26/39 years and according to Chi-square test it was determined that age attribution , delivery numbers , educational level , pregnancy age , abortion numbers and pregnant mothers jobs in three groups are nearly similar to each other . The results showed that the min amount of bleeding of third stage of delivery from low to high was (167/147)±306/7) in the physiologically managed group , (220/21)±321/20 in oxytocin ejection group and (219/71)±329/2 and the min of the length of third stage of delivery from low to high was (4/91)±8/83 in the nipple stimulation group , (5/54)±10/42 in oxytocin ejection group and 2(8/07)±11/20 in physiologically managed group . And these differences was not statistically significant (p=0/821 وp=0/162) . conclusion : As there was not any impressive and meaningful difference in distinction of average bleeding of third stage of delivery and the length of period in these groups ,it seems that using oxytocin injection ,does not have any positives effect on the amount of bleeding of the third stage of delivery.