INTRODUCTION
Artichoke is a perennial plant; its scientific name is Gundelia tournefortii L., and it belongs to the Asteraceae (Compositae) family. Artichoke is one of the most abundant plants in the regions of Iran, where it is easily propagated in nature and grows abundantly in all mountainous areas of Iran and the Mediterranean (Farhang et al.,2016). In traditional medicine and ethnobotany, this plant has nutritional and therapeutic benefits for diabetes, epilepsy, as well as stomach and intestinal diseases (Özaltun et al.,2019). Moreover, the antioxidant, liver protection, and antibacterial effects of this plant have been confirmed (Asadi et al.,2013). Nowadays, the artichoke plant, with its high antioxidant and antitumor properties, is considered to destroy cancer cells.

METHODS
To perform the experiment, the 60-HL cell line was obtained from Pasteur Institute of Iran, and the cells were cultured in RPMI culture medium containing 10% FBS and 1% penicillin/streptomycin in RM11640 culture medium under 5% CO₂ pressure and temperature of 37°C. In this experimental study, artichoke stems were collected in the study area for extraction and dried separately at ambient temperature, and the dried plant was ground by a grinder. 60-HL cells were treated with different concentrations of 1, 5, 10, 20, and 40 μg/ml artichoke extract for 48 h ( Amer et al,2022).

RESULTS
The results obtained from the MTT assay indicated that the artichoke plant extract from the Nahrab region, at a concentration of 20 µg/ml over 48 h, killed 50% of the cells. In contrast, the artichoke plant extracts from the Ghori Qala and Khanum Abad regions, at a concentration of 10 µg/ml over 48 h, also killed 50% of the cells. The plant extracts from all three regions at a 1 µg/ml concentration did not exhibit a significant cytotoxic effect on cancer cells. However, the artichoke plant extract from the Ghori Qala and Khanum Abad regions at concentrations of 0.2 and 40 µg/ml, as well as the artichoke plant extract from the Nahrab region at a concentration of 40 µg/ml, were considerably lethal to cancer cells.

CONCLUSION
The results of the present study indicated that the artichoke plant extract extracted from the Ghori Qala and Khanum Abad regions has a more substantial effect on killing cancer cells compared to the plant extract extracted from the Nahrab region, and it seems that the anticancer compounds in the artichoke plants from the Nahrab region are more abundant, as there are more artichoke plants in that area. Meanwhile, the plant extracts obtained from the Ghori Qala and Khanum Abad regions did not show significant differences.

Ethical Considerations
Compliance with ethical guidelines

Funding
There is no funding support.

Authors' Contributions
The authors contributed equally to the conceptualization and writing of the article. All of the authors approved the content of the manuscript and agreed on all aspects of the work.

Conflict of Interest
The authors declared that there is no conflict of interest.

Acknowledgments
We are grateful to everyone who provided scientific consulting for this work.