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Volume 10, Issue 3 (12-2020)                   cmja 2020, 10(3): 206-217 | Back to browse issues page


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Zarghami Khameneh A, Jafari A, Nikookheslat S, Karimi P. Effect of Chronic Caffeine Administration on Expression Ratio of Bax and Bcl-2 Proteins in Myocardial Tissue of Male Wistar Rats With Type 2 Diabetes. cmja. 2020; 10 (3) :206-217
URL: http://cmja.arakmu.ac.ir/article-1-737-en.html
1- Department of Sport Physiology, Faculty of Physical Eduction and Sport Sciences, University of Tabriz, Tabriz, Iran.
2- Department of Sport Biological Sciences, Faculty of Sport Sciences and health, Shahid Beheshti University, Tehran, Iran. , af_jafari@sbu.ac.ir
3- Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract:   (578 Views)
Objective: Some previous studies have shown the protective effect of caffeine on apoptosis through the regulation of pro- and anti-apoptotic proteins. The aim of this study was to investigate the effects of chronic caffeine administration on the expression ratio of B-cell lymphoma protein 2 (Bcl-2) and Bcl-2-Associated X-protein (Bax) proteins in the cardiac tissue of rats with Type 2 Diabetes.
Methods: In this experimental study, samples were 24 male white wistar rats (aged 2-3 months with a weight of 250-300 g) randomly divided into three groups: Healthy control (n=8), untreated diabetic (n=8), and diabetic with caffeine supplement (n=8; 70 mg/kg-1 for 8 weeks, 5 days a week). The expression of proteins associated with apoptotic signaling pathway (Bax and Bcl-2) in the cardiac muscle (left ventricular) was measured by Western blot method. One-Way Variance (ANOVA), t-test, and Tukey’s post hoc test were used for data analysis.
Results: Induction of type two diabetes significantly increased the expression of Bax protein (1.81±0.2) and decreased the expression of Bcl-2 protein (0.36±0.05) compared to control group (P=0.001). However, caffeine administration increased the expression of Bax protein (131%) compared to diabetic control group (P=0.001). Therefore, caffeine administration after diabetes induction elevated the  Bax/Bcl-2 ratio (P=0.001).
Conclusion: Eight weeks of caffeine administration have an exacerbating effect on the apoptotic cell death caused by type 2 diabetes by increasing pro-apoptotic proteins and reducing anti-apoptotic proteins.
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Type of Study: Research | Subject: Physiology

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