Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

Heydari, Bahare; Ghofrani, Mohsen; Bahram, Mohammad Ebrahim

doi:10.32598/cmja.10.4.631.2

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Volume 10, Issue 4 (3-2021) cmja 2021, 10(4): 328-339 | Back to browse issues page

‎ 10.32598/cmja.10.4.631.2

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Heydari B, Ghofrani M, Bahram M E. Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players. cmja 2021; 10 (4) :328-339
URL: http://cmja.arakmu.ac.ir/article-1-773-en.html

Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

Bahare Heydari¹

, Mohsen Ghofrani¹

, Mohammad Ebrahim Bahram

1- Department of Physical Education and Sport Sciences, Faculty of Educational Sciences and Psychology, University of Sistan and Baluchestan, Zahedan, Iran.
2- Department of Physical Education and Sport Sciences, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran. , bahramsport2010@gmail.com

Keywords: Massage, Malondialdehyde, Glutathione peroxidase, Superoxide dismutase, Exhaustive exercise

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1. Introduction

alondialdehyde (MDA) is a small but stable product of the lipid peroxidation that is resulted from the breakdown of unstable peroxides of unsaturated fatty acids [3] Given that intense exercise causes oxidative stress, cells use the enzymatic antioxidant defense system including Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) as the first line of defense against oxidative stress. Massage includes a group of manual techniques on body tissues to affect the neuromuscular, skin, and joints systems, reduce stress and pain, and improve the blood and lymphatic circulation. It has long been used as a complementary method for the treatment and improvement of body function and the prevention of injuries [10].
To our knowledge, no studies have been conducted on the effect of massage after exercise on the MDA, SOD and GPX markers. However, there are some studies on the effect of various massages on the markers of muscle injury, oxidation, anti-oxidation, and immune system. Yousefi et al. showed that massage after intense physical activity had a positive effect on immunoglobulin A and neutrophils in athletes with disabilities [14]. Naghizadeh et al. reported that the serum levels of creatinine kinase and lactate dehydrogenase slightly increased after massage compared to an eccentric exercise session [15]. Sadat and Hosseinizadeh in a study investigated the effect of sport massage on hematological parameters in semi-professional male runners. Their results showed a significant difference between the two types of active and sports massage in white blood cell, hemoglobin and hematocrit levels [16]. It has also been reported that massage has no effect on the strength and athletic performance and may even cause a decline in athletic performance [19]. Considering the importance of the massage, the present study aims to evaluate the effect of three types of massage on serum levels of MDA, SOD and GPX following a session of exhaustive exercise.

2. Materials and Methods

This is a quasi-experimental study conducted on 48 female futsal players aged 17-22 years in Zahedan, Iran during 2019-2020, who were voluntarily participated and randomly divided into four groups: Swedish massage, Russian massage, Thai massage, and control. Five cc of fasting blood was taken from the anterior brachial vein of subjects three times simultaneously from 8 to 10 A.M. To examine the difference within groups, repeated measures ANOVA was used and to measure differences between groups, one-way ANOVA was carried out. The significance level was set at 0.05.

3. Results

As shown in Table 1, the results of repeated measures ANOVA showed that massage after exhaustive exercise leads to a significant reduction in MDA (F=73.811, P=0.001), GPX (F=44.237, P=0.001) and SOD (F=14.594, P=0.001) levels. The results of one-way ANOVA showed a significant difference between the effects of three types of sports massage on serum levels of MDA, SOD and GPX after one session of exhaustive exercise (P=0.001).

4. Conclusion

This study aimed to investigate the effect of three types of massage on serum levels of MDA, SOD and GPX following a session of exhaustive exercise. To the best of our knowledge, there is no other similar study to compare our findings with their results. Yousefi and Azamian in a study showed that 20 minutes of effleurage sports massage can have a beneficial effect on the immune system of wheelchair basketball players [14]. This is consistent with our results. There is a powerful antioxidant called cytochrome that recycles reactive oxygen species and produces water by transferring electrons to oxygen; it reduces electron emission and, thus, the production of reactive oxygen species, and electron leakage [22].
The findings of the present study are against the results of Ang et al. and Kim et al. [24, 25]. This discrepancy may be due to the type and technique of applied massage. Consistent with the present study, Piri et al. showed that massage therapy leads to a significant reduction in oxidation markers by 25% [26]. Against the present study, Billhall et al. showed that massage therapy has no effect on biochemical markers [32]. Due to impaired balance between the oxygen consumption and the oxygen required by the involved tissues and the creation of a process called ischemia, exhaustive exercise seems to cause damage to unsaturated lipids in tissue membranes and production of reactive oxygen species, which in turn stimulates lipid peroxidation and ultimately increases the production of free radicals.
It seems that the use of massage during the recovery period and rest after exhaustive exercise can reduce the level of MDA, as an oxidative stress marker, and increase oxidative enzymes of SOD and GPX.

Ethical Considerations

Compliance with ethical guidelines

This study was approved by the University of Sistan and Baluchestan (Code: IR.US.Rec.1399.31409). All participants in this study signed a written consent form and were assured of the confidentiality of their information.

Funding

This study was extracted from the MA. thesis of first author at the Department of Physical Education and Sports Sciences, University of Sistan and Baluchestan.

Authors' contributions

All authors contributed equally in preparing this article.

Conflicts of interest

The authors declared no conflict of interest.

Type of Study: Research | Subject: Physiology

References

1. [1] Alikhani S & Sheikholeslami-Vatani D. Oxidative stress and anti-oxidant responses to regular resistance training in young and older adult women. GGI Journal. 2019; 19(5): 419-22. https://doi.org/10.1111/ggi.13636 [DOI:10.1111/ggi.13636.] [PMID]

2. [2] McLeay Y, Stannard S, Houltham S, Starck C. Dietary thiols in exercise: oxidative stress defence, exercise performance, and adaptation. J Int Soc Sports Nutr. 2017; 14(1):12-27. https://doi.org/10.1186/s12970-017-0168-9 [DOI:10.1186/s12970-017-0168-9.] [PMID] [PMCID]

3. [3] Thirumalai T, Therasa SV, Elumalai EK and David E. Intense and exhaustive exercise induce oxidative stress in skeletal muscle. Asian Pac J Trop Dis. 2011; 1(1):63-66. https://doi.org/10.1016/S2222-1808(11)60016-9 [DOI:10.1016/S2222-1808 (11)60016-9]

4. [4] Belviranl M, Gökbel H, Okudan N and Başaralı K. Effects of grape seed extract supplementation on exercise-induced oxidative stress in rats. Br J Nutr. 2012; 108(2):249-56. [DOI:10.1017/S0007114511005496] [PMID]

5. [5] Ahmadi-kakavandi M, Azizbeigi K, Qeysari SF. The Effects of Progressive Resistance Training on Malondialdehyde Concentration and Superoxide Dismutase Enzyme Activity in Inactive Elderly Women. payavard. 2019; 13 (2):151-159. [In Persian] http://payavard.tums.ac.ir/article-1-6764-en.html

6. [6] Li S, Tan H, Wang N, Zhang Z, Lao L, Wong .The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int J Mol Sci. 2015; 16)11(: 26087-124. [DOI:10.3390/ijms161125942] [PMID] [PMCID]

7. [7] Kostaropoulosia IA, Nikolaidis MG, Jamurtas AZ, Ikonomou GV, Makrygiannis V, Papadopoulos G,. Kouretas D. Comparison of the Blood Redox Status Between Long Distance and Short Distance Runners. Physiol Res. 2006;1 )55(: 611-16.

8. [8] Bas DG, Elsa H, Nicolas F, Inge M, Romain M. Effects active and passive recovery on electro stimulation and maximal climbing performance. Annual congress of the ECSS Jyvaskyla, Finland. 2007; 11-14. http://journals.hsu.ac.ir/sbs/article-۱-۱۲۲-fa.html

9. [9] Hilbert JE, Asforzo G, Swensen T. Effect of massage on delayed onset muscle soreness. Br J Sports Med. 2003; 37(1):72-75. http://dx.doi.org/10.1136/bjsm.37.1.72 [DOI:10.1136/bjsm.37.1.72] [PMID] [PMCID]

10. [10] Buttagat V, Eungpinichpong W, Chatchawan U, Kharmwan S. The immediate effects of traditional Thai massage on heart rate variability and stress-related parameters in patients with back pain associated with myofascial trigger points. J Bodyw Mov Ther. 2011; 15(1): 15-23. [DOI:10.1016/j.jbmt.2009.06.005] [PMID]

11. [11] Pinar S., Kaya F., Bicer B., Erzeybek M.S., Cotuk H.B. Different recovery methods and muscle performances after exhausting exercise: comparison of the effects of electrical muscle stimulation and massage. Biol. Sport. 2012; 29(4): 269-75. [DOI:10.5604/20831862.1019664] [PMID] [PMCID]

12. [12] Weerapong P, Hume PA, Kolt GS. The mechanisms of massage and effects on performance, muscle recovery and injury prevention. Sports Med. 2005; 35(3): 235-56. [DOI:10.2165/00007256-200535030-00004] [PMID]

13. [13] Karabulut AB, Kafkas ME, Kafkas AS, Onal Y, Kiran TR. The effect of regular exercise and massage on oxidant and antioxidant parameters. Indian J Physiol Pharmacol. 2013; 57(4):378-83.

14. [14] Yousefi Saghazi S, Azamian Jazi A. The effect of caressing sports massage on some safety indicators of elite basketball players in wheelchairs after a hard training session. Sports Medicine.2015; 7(2):267-78. [In Persian] 10.22059/JSMED.2015.56565

15. [15] Emani E, MoshtaghEshgh Z, Hosseini T, Alavi Majd H, AbedSaeidi Zh. The effect of foot massage on physiological indicators of female patients with CVA admitted in the ICU. J Shahid Sadoughi UMS.2009; 17(2 65):76-82. [Persian] http://jssu.ssu.ac.ir/article-1-1369-en.html

16. [16] Saadat F, Hossienzadeh M. The Effect of Sports Massage on Hematological Parameters in Semi-Professional Male Runners. Report of Health Care.2019; 5(3): 8-13. [In Persian] http://jrhc.miau.ac.ir/article_3501.html

17. [17] Poorbarzegar M, Minoonejad H, Seidi F, Mozafaripour E. The immediate effect of sports massage on proprioception of knee and ankle joints in collegiate male athletes. SJKU.2017;21(6):72-82. [In Persian] http://sjku.muk.ac.ir/article-1-2825-en.html

18. [18] Ilbeigi S, Ayubi Avaz M, Saghebjoo M, zardast M. Acute effects of proprioception, massage and dynamic stretching warm up protocols on serum CK and LDH activity levels after one session of Plyometric training in male volleyball players. Koomesh.2016;17(2):393-402. [In Persian] http://koomeshjournal.semums.ac.ir/article-1-2762-en.html

19. [19] Hunter AM, Watt JM, Watt V, Galloway SDR. Effect of lower limb massage on electromyography and force

20. production of the knee extensors. Br J Sports Med. 2006;40(2):114-8. http://dx.doi.org/10.1136/bjsm.2005.019075 [DOI:10.1136/bjsm.2005.019075] [PMID] [PMCID]

21. [20] Fattahi Bafghi A, Homaee H M, Azarbayjani M A. Effects of High Intensity Interval Training and Curcumin Supplement on Antioxidant Enzyme in Heart Tissue of Diabetic Rats. IJDO. 2016; 8 (3):135-41. [In Persian] http://ijdo.ssu.ac.ir/article-1-309-en.html

22. [21] Bahram ME, Ghofrani M, Pourvaghar M J, Arabpour H. Comparing the Effect of Wet Cupping with that of Intensive Aerobic Workout on HDL and LDL Levels in Young Male Athletes. C m j a. 2018; 8 (3):2330-340. [In Persian] http://cmja.arakmu.ac.ir/article-1-442-en.html

23. [22] Parise G, Phillips SM, Kaczor JJ & Tarnopolsky MA. Antioxidant enzyme activity is up-regulated after unilateral resistance exercise training in older adults. Free Radical Biology and Medicine. 2005; 39(2): 289-95. 10.1016/j.freeradbiomed.2005.03.024 [DOI:10.1016/j.freeradbiomed.2005.03.024] [PMID]

24. [23] Hernandez-Reif M, Ironson G, Field T, Hurley J, Katz G, Diego M, Weiss S, Fletcher MA, Schanberg S, Kuhn C, Burman I.2004. Breast cancer patients have improved immune and neuroendocrine functions following massage therapy .J Psychosomatic Res. 2004; 57(1): 45-52. [DOI:10.1016/S0022-3999(03)00500-2]

25. [24] Ang JY, Lua JL, Mathur A, Thomas R, Asmar BI, Savasan S, Buck S, Long M, Shankaran S. A randomized placebo-controlled trial of massage therapy on the immune system of preterm infants. Pediatrics. 2012; 130(6): 1549-58. DOI: [DOI:10.1542/peds.2012-0196] [PMID] [PMCID]

26. [25] Kim JO. Kim IS. Effects of aroma self-foot reflexology massage on stress and immune responses and fatigue in middle-aged women in rural areas. Journal of Korean Acad Nurs. 2012;42(5): 709-18. DOI: [DOI:10.1542/peds.2012-0196] [PMID] [PMCID]

27. [26] Piri M, Sheikh- Sarraf, B, Azarbayjani, M-A, Agha Ali Nejad, H. The effect of 8 weeks of periodic aerobic exercise and massage therapy on C-reactive protein changes and cardiovascular fitness of cardiovascular patients after open heart surgery. Journal of Applied Sports Physiology. 50-61, 10 (19): 2014. DOI: 10.7508/aass.2016.01.003

28. [27] Samjoo IA, Safdar A, Hamadeh MJ, Raha S & Tarnopolsky MA. The effect of endurance exercise on both skeletal muscle and systemic oxidative stress in previously sedentary obese men. Nutr Diabetes. 2013; 3(9):88. [DOI:10.1038/nutd.2013.30] [PMID] [PMCID]

29. [28] Zainuddin Z, Newton M, Sacco P, Nosaka K. Effects of massage on delayed- onset muscle soreness, swelling, and recovery of muscle function. J Athl Train. 2005; 40(3): 174-80. PMID: 16284637

30. [29] Petibois C & Deleris G. Erythrocytes adaptation to oxidative stress in endurance training. Arch Invest Med. 2005; 36(5): 524-31. [DOI:10.1016/j.arcmed.2005.03.047] [PMID]

31. [30] Ceci R, Beltran-Valls MR, Duranti G, Dimauro I, Quaranta F, Pittaluga M, et al. Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training. Redox Biology. 2014; 2(1): 65-72. [DOI:10.1016/j.redox.2013.12.004] [PMID] [PMCID]

32. [31] Vezzoli A, Pugliese L, Marzorati M, Serpiello FR, La Torre A & Porcelli S. Time-course changes of oxidative stress response to high-intensity discontinuous training versus moderate-intensity continuous training in masters runners. PLoS One. 2014; 9(1): 87506. [DOI:10.1371/journal.pone.0087506] [PMID] [PMCID]

33. [32] Billhult A, Lindholm C, Gunnarsson R, Stener-Victorin E. The effect of massage on immune function and stress in women with breast cancer - A randomized controlled trial. Auton Neurosci: Basic Clin. 2009; 7 (5):111-5. [DOI:10.1016/j.autneu.2009.03.010] [PMID]

34. [33] Dabidi Roshan V. Moslehi-Najafabadi E. The Effect of short-term vitamin E supplementation on some indexes of sport performances and lipid per-oxidation in healthy men. World J Sport. 2009; 2(2): 75-81. http://idosi.org/wjss/wjss2(2)09.htm

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