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Volume 10, Issue 4 (3-2021)
cmja 2021, 10(4): 396-409 |
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Shirkhani Y, Peeri M, Azarbayjani M A, Matinhomaee H. Effect of Resistance Exercise and Vitamin C Intake on Expression of Telomerase Reverse Transcriptase and Telomere Repeat Binding Factor-2 Genes and the Diameter and Number of Myofibrils in Old Rats. cmja 2021; 10 (4) :396-409
URL: http://cmja.arakmu.ac.ir/article-1-789-en.html
URL: http://cmja.arakmu.ac.ir/article-1-789-en.html
1- Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran. , m.peeri@gmail.com
2- Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran. , m.peeri@gmail.com
Abstract: (2526 Views)
Objective: Cell aging is one of the most important and fundamental step in cellular behavior and reduces muscle mass and myofibrils. This study aims to investigate the effect of resistance exercise along with vitamin C consumption on the expression of Telomerase Reverse Transcriptase (TERT) and Telomere Repeat Binding Factor-2 (TRF2) genes and the diameter and number of skeletal muscle myofibrils in old male Wistar rats.
Methods: This is an experimental study using control groups. Twenty-five male Wistar rats (280-320 g and aged 24 weeks) were randomly divided into five groups of young control, old+resistance exercise, old+vitamin intake, old+resistance exercise+vitamin intake, and old control. In the supplementation groups, rats received liposomal vitamin C daily by gavage per body weight. Weight attached to the tail gradually increased during 8 weeks of exercise (weeks 1 and 2 by 50%; weeks 3 and 4 by 50%; weeks 5 and 6 by 75%; weeks 7 and 8 by 100% of total body weight). Exercises were performed at 3-5 sets of 8-12 repetitions, 3 or 4 days a week. The Quantitative polymerase chain reaction method was used to evaluate the expression of TERT and TRF2 genes in muscle tissue. One-way ANOVA was used to examine the difference between the groups and Tukey’s post hoc test was used to determine between which groups the difference was significant.
Results: Aging significantly reduced the expression of TERT, TRF2 genes and the diameter and number of myofibrils in skeletal muscle of rats (P=0.001). Resistance exercise along with vitamin C intake had no significant effect on TERT and TRF2 expression (P≥0.05), but caused a significant increase in the diameter and number of myofibrils (P= 0.001).
Conclusion: Eight weeks of resistance exercise along with vitamin C supplementation can significantly increase the number and diameter of skeletal muscle myofibrils in old rats.
Methods: This is an experimental study using control groups. Twenty-five male Wistar rats (280-320 g and aged 24 weeks) were randomly divided into five groups of young control, old+resistance exercise, old+vitamin intake, old+resistance exercise+vitamin intake, and old control. In the supplementation groups, rats received liposomal vitamin C daily by gavage per body weight. Weight attached to the tail gradually increased during 8 weeks of exercise (weeks 1 and 2 by 50%; weeks 3 and 4 by 50%; weeks 5 and 6 by 75%; weeks 7 and 8 by 100% of total body weight). Exercises were performed at 3-5 sets of 8-12 repetitions, 3 or 4 days a week. The Quantitative polymerase chain reaction method was used to evaluate the expression of TERT and TRF2 genes in muscle tissue. One-way ANOVA was used to examine the difference between the groups and Tukey’s post hoc test was used to determine between which groups the difference was significant.
Results: Aging significantly reduced the expression of TERT, TRF2 genes and the diameter and number of myofibrils in skeletal muscle of rats (P=0.001). Resistance exercise along with vitamin C intake had no significant effect on TERT and TRF2 expression (P≥0.05), but caused a significant increase in the diameter and number of myofibrils (P= 0.001).
Conclusion: Eight weeks of resistance exercise along with vitamin C supplementation can significantly increase the number and diameter of skeletal muscle myofibrils in old rats.
Keywords: Telomerase Reverse Transcriptase, Telomerase Repeat Binding Factor 2, Aging, Resistance exercise, Vitamin C
Type of Study: Research |
Subject:
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References
1. Miljkovic N, Lim J-Y, Miljkovic I, Frontera WR. Aging of skeletal muscle fibers. Annals of rehabilitation medicine. 2015;39(2):155. [DOI:10.5535/arm.2015.39.2.155] [PMID] [PMCID]
2. Morsiani C, Bacalini MG, Santoro A, Garagnani P, Collura S, D'Errico A, et al. The peculiar aging of human liver: A geroscience perspective within transplant context. Ageing research reviews. 2019;51:24-34. [DOI:10.1016/j.arr.2019.02.002] [PMID]
3. Ho RT, Chan JS, Wang C-W, Lau BW, So KF, Yuen LP, et al. A randomized controlled trial of qigong exercise on fatigue symptoms, functioning, and telomerase activity in persons with chronic fatigue or chronic fatigue syndrome. Annals of Behavioral Medicine. 2012;44(2):160-70. [DOI:10.1007/s12160-012-9381-6] [PMID] [PMCID]
4. Pejenaute Á, Cortés A, Marqués J, Montero L, Beloqui Ó, Fortuño A, et al. NADPH oxidase overactivity underlies telomere shortening in human atherosclerosis. International Journal of Molecular Sciences. 2020;21(4):1434. [DOI:10.3390/ijms21041434] [PMID] [PMCID]
5. Heidenreich B, Kumar R. TERT promoter mutations in telomere biology. Mutation Research/Reviews in Mutation Research. 2017;771:15-31. [DOI:10.1016/j.mrrev.2016.11.002] [PMID]
6. Slattery ML, Herrick JS, Pellatt AJ, Wolff RK, Mullany LE. Telomere length, TERT, and miRNA expression. PLoS One. 2016;11(9):e0162077. [DOI:10.1371/journal.pone.0162077] [PMID] [PMCID]
7. Monaghan P, Eisenberg DT, Harrington L, Nussey D. Understanding diversity in telomere dynamics. The Royal Society; 2018. [DOI:10.1098/rstb.2016.0435] [PMID] [PMCID]
8. Prasad KN, Wu M, Bondy SC. Telomere shortening during aging: Attenuation by antioxidants and anti-inflammatory agents. Mechanisms of ageing and development. 2017;164:61-6. [DOI:10.1016/j.mad.2017.04.004] [PMID]
9. de Vos-Houben JM, Ottenheim NR, Kafatos A, Buijsse B, Hageman GJ, Kromhout D, et al. Telomere length, oxidative stress, and antioxidant status in elderly men in Zutphen and Crete. Mechanisms of ageing and development. 2012;133(6):373-7. [DOI:10.1016/j.mad.2012.04.003] [PMID]
10. Kiecolt-Glaser JK, Epel ES, Belury MA, Andridge R, Lin J, Glaser R, et al. Omega-3 fatty acids, oxidative stress, and leukocyte telomere length: a randomized controlled trial. Brain, behavior, and immunity. 2013;28:16-24. [DOI:10.1016/j.bbi.2012.09.004] [PMID] [PMCID]
11. Bruns DR, Ehrlicher SE, Khademi S, Biela LM, Peelor III FF, Miller BF, et al. Differential effects of vitamin C or protandim on skeletal muscle adaptation to exercise. Journal of Applied Physiology. 2018;125(2):661-71. [DOI:10.1152/japplphysiol.00277.2018] [PMID] [PMCID]
12. Negaresh R, Ranjbar R, Habibi A, Gharibvand MM. The relationship between muscle volume and strength and some factors associated with sarcopenia in old men compared with young men. Zanko Journal of Medical Sciences. 2016;17(54):23-34.
13. Harris SE, Deary IJ, MacIntyre A, Lamb KJ, Radhakrishnan K, Starr JM, et al. The association between telomere length, physical health, cognitive ageing, and mortality in non-demented older people. Neuroscience letters. 2006;406(3):260-4. [DOI:10.1016/j.neulet.2006.07.055] [PMID]
14. Jiang H, Schiffer E, Song Z, Wang J, Zürbig P, Thedieck K, et al. Proteins induced by telomere dysfunction and DNA damage represent biomarkers of human aging and disease. Proceedings of the National Academy of Sciences. 2008;105(32):11299-304. [DOI:10.1073/pnas.0801457105] [PMID] [PMCID]
15. Negaresh R, Ranjbar R, Habibi A, Gharibvand MM. The effects of eight weeks of resistance training on some muscle hypertrophy and physiological parameters in elderly men. Journal of Geriatric Nursing. 2016;3(1):62-75. [DOI:10.21859/jgn.3.1.62]
16. Rae DE, Vignaud A, Butler-Browne GS, Thornell L-E, Sinclair-Smith C, Derman EW, et al. Skeletal muscle telomere length in healthy, experienced, endurance runners. European journal of applied physiology. 2010;109(2):323-30. [DOI:10.1007/s00421-010-1353-6] [PMID]
17. Ponsot E, Lexell J, Kadi F. Skeletal muscle telomere length is not impaired in healthy physically active old women and men. Muscle & nerve. 2008;37(4):467-72. [DOI:10.1002/mus.20964] [PMID]
18. Werner C, Fürster T, Widmann T, Pöss J, Roggia C, Hanhoun M, et al. CLINICAL PERSPECTIVE. Circulation. 2009;120(24):2438-47. [DOI:10.1161/CIRCULATIONAHA.109.861005] [PMID]
19. Ludlow AT, Witkowski S, Marshall MR, Wang J, Lima LC, Guth LM, et al. Chronic exercise modifies age-related telomere dynamics in a tissue-specific fashion. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2012;67(9):911-26. [DOI:10.1093/gerona/gls002] [PMID] [PMCID]
20. Eskandari A, Fashi M, Dakhili AB. Effect of high intensity interval and continuous endurance training on TRF2 and TERT gene expression in heart tissue of aging male rats. Journal of Gorgan University of Medical Sciences. 2019;21(2).
21. Thirupathi A, da Silva Pieri BL, Queiroz JAMP, Rodrigues MS, de Bem Silveira G, de Souza DR, et al. Strength training and aerobic exercise alter mitochondrial parameters in brown adipose tissue and equally reduce body adiposity in aged rats. Journal of physiology and biochemistry. 2019;75(1):101-8. [DOI:10.1007/s13105-019-00663-x] [PMID]
22. Scheffer DL, Silva LA, Tromm CB, da Rosa GL, Silveira PC, de Souza CT, et al. Impact of different resistance training protocols on muscular oxidative stress parameters. Applied Physiology, Nutrition, and Metabolism. 2012;37(6):1239-46. [DOI:10.1139/h2012-115] [PMID]
23. Damas F, Phillips SM, Libardi CA, Vechin FC, Lixandrão ME, Jannig PR, et al. Resistance training‐induced changes in integrated myofibrillar protein synthesis are related to hypertrophy only after attenuation of muscle damage. The Journal of physiology. 2016;594(18):5209-22. [DOI:10.1113/JP272472] [PMID] [PMCID]
24. Gabrial SG, Shakib M-CR, Gabrial GN. Protective Role of Vitamin C Intake on Muscle Damage in Male Adolescents Performing Strenuous Physical Activity. Open access Macedonian journal of medical sciences. 2018(6(9:1594. [DOI:10.3889/oamjms.2018.337] [PMID] [PMCID]
25. Haendeler J, Hoffmann Jr, Diehl JF, Vasa M, Spyridopoulos I, Zeiher AM, et al. Antioxidants inhibit nuclear export of telomerase reverse transcriptase and delay replicative senescence of endothelial cells. Circulation research. 2004;94(6):768-75. [DOI:10.1161/01.RES.0000121104.05977.F3] [PMID]
26. Makino N, Maeda T, Oyama J-i, Sasaki M, Higuchi Y, Mimori K, et al. Antioxidant therapy attenuates myocardial telomerase activity reduction in superoxide dismutase-deficient mice. Journal of molecular and cellular cardiology. 2011;50(4):670-7. [DOI:10.1016/j.yjmcc.2010.12.014] [PMID]
27. Rose BA, Force T, Wang Y. Mitogen-activated protein kinase signaling in the heart: angels versus demons in a heart-breaking tale. Physiological reviews. 2010;90(4):1507-46. [DOI:10.1152/physrev.00054.2009] [PMID] [PMCID]
28. Baghaiee B, Karimi P, Siahkouhian M, Pescatello LS. Moderate aerobic exercise training decreases middle-aged induced pathologic cardiac hypertrophy by improving Klotho expression, MAPK signaling pathway and oxidative stress status in Wistar rats. Iranian Journal of Basic Medical Sciences. 2018;21(9):911-9.
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