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Seyyedeh Maryam Zamanian Dehkordi1 
, Mohammad Zavarshani2 , Mohsen Jafarian Dehkordi *3 , Mohsen Asgari4 , Maryam Zavarshani4
, Mohammad Zavarshani2 , Mohsen Jafarian Dehkordi *3 , Mohsen Asgari4 , Maryam Zavarshani4
1- Doctor of Veterinary Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Shahrekord, Shahrekord, Iran
2- PhD in Bacteriology, Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
3- Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Shahrekord, Shahrekord, Iran ,mjafariand@yahoo.com
4- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
2- PhD in Bacteriology, Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
3- Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Shahrekord, Shahrekord, Iran ,
4- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
Abstract: (23 Views)
Introduction: Glycated hemoglobin (HbA1c) is considered one of the most important indicators for long-term glycemic control and progression of diabetic complications. The present study aimed to investigate the effect of the hydroalcoholic extract of Pistacia atlantica fruit on HbA1c formation in human blood samples under in vitro conditions.
Methods: In this in vitro study, human blood samples were exposed to glucose concentrations of 20 and 40 mM and treated with different concentrations (0.1, 0.5, and 1 g/dL) of the hydroalcoholic extract of Pistacia atlantica fruit. HbA1c levels were measured after 7 and 14 days of incubation using a colorimetric method, and the results were compared with those of the positive control group. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test, with a significance level set at P<0.05.
Results: The results demonstrated that treatment of human blood samples with the hydroalcoholic extract of Pistacia atlantica under hyperglycemic conditions (20 and 40 mM glucose) led to a statistically significant reduction in HbA1c levels compared with the positive control group (P<0.05). This reduction was observed at both incubation time points, with a more pronounced inhibitory effect on day 14 compared to day 7. The greatest decrease in HbA1c was consistently observed at the extract concentration of 0.1 g/dL across all experimental conditions. In contrast, higher extract concentrations exhibited a reduced inhibitory effect, and in some conditions, a relative increase in HbA1c levels compared to the positive control was observed. Overall, the findings of this study indicated that the inhibitory effect of the hydroalcoholic extract of Pistacia atlantica on HbA1c formation was dependent on both dose and incubation time.
Conclusion: The findings of the present work indicated that the hydroalcoholic extract of Pistacia atlantica fruit possessed the potential to inhibit HbA1c formation under in vitro conditions and could exert a role in the prevention or attenuation of diabetes-related complications through the suppression of non-enzymatic glycation. However, further studies are required to clarify the clinical relevance of these effects.
Methods: In this in vitro study, human blood samples were exposed to glucose concentrations of 20 and 40 mM and treated with different concentrations (0.1, 0.5, and 1 g/dL) of the hydroalcoholic extract of Pistacia atlantica fruit. HbA1c levels were measured after 7 and 14 days of incubation using a colorimetric method, and the results were compared with those of the positive control group. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test, with a significance level set at P<0.05.
Results: The results demonstrated that treatment of human blood samples with the hydroalcoholic extract of Pistacia atlantica under hyperglycemic conditions (20 and 40 mM glucose) led to a statistically significant reduction in HbA1c levels compared with the positive control group (P<0.05). This reduction was observed at both incubation time points, with a more pronounced inhibitory effect on day 14 compared to day 7. The greatest decrease in HbA1c was consistently observed at the extract concentration of 0.1 g/dL across all experimental conditions. In contrast, higher extract concentrations exhibited a reduced inhibitory effect, and in some conditions, a relative increase in HbA1c levels compared to the positive control was observed. Overall, the findings of this study indicated that the inhibitory effect of the hydroalcoholic extract of Pistacia atlantica on HbA1c formation was dependent on both dose and incubation time.
Conclusion: The findings of the present work indicated that the hydroalcoholic extract of Pistacia atlantica fruit possessed the potential to inhibit HbA1c formation under in vitro conditions and could exert a role in the prevention or attenuation of diabetes-related complications through the suppression of non-enzymatic glycation. However, further studies are required to clarify the clinical relevance of these effects.
Type of Study: Research |
Subject:
Medicinal Plants
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