Introduction: Hyperlipidemia is one of the modifiable risk factors for cardiovascular diseases. The primary study focused on the effects of a combination of Amaranthus hybridus leaf extract and Glycyrrhiza glabra root extract on lipid profiles. The present analysis aimed to investigate the pattern of lipid profile components according to temperament, with emphasis on age and sex, in patients with hyperlipidemia.
Methods: This study was a secondary analysis of baseline data from a clinical trial. Among 65 patients, 63 had temperament records that could be standardized. Temperament was classified using the 10-item Mojahedi questionnaire along two dimensions: hot-cold and wet-dry. TC, TG, LDL-C, and HDL-C were analyzed according to the criteria defined in the original study. Statistical tests appropriate to the type and distribution of the data included Fisher’s exact test, Mann-Whitney U test, Kruskal-Wallis test, and Spearman correlation, and multiple linear regression adjusted for age and sex was used for adjusted analysis. 
Results: In the total sample, the “cold and wet” temperament was the most prevalent combined temperament, observed in 18 patients (27.7%). Among the 63 patients with definitive temperament classification, 55.6% were classified as cold and 50.8% as wet. The hot-cold dimension was significantly associated with sex (p=0.002) and age group (p=0.023), but showed no significant differences in lipid parameters. In the wet-dry dimension, significant differences were observed in TC (p=0.004) and LDL-C (p=0.014), with the dry group showing the highest values. In addition, age showed a positive correlation with TC, with a correlation coefficient of 0.354 and a significance level of 0.004, and with LDL-C, with a correlation coefficient of 0.361 and a significance level of 0.003. HDL-C also differed significantly by sex (significance level=0.024).
Conclusion: In this sample, differences in lipid parameters were more evident along the wet-dry dimension, with the dry group showing higher TC and LDL-C levels. These findings represent observational associations and do not establish a causal relationship between temperament and hyperlipidemia.