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Volume 16, Issue 2 (8-2026)
cmja 2026, 16(2): 0-0 |
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Nasr Esfahani F, Azizi Farsani F, Soltani Fard E, Rahimi Madiseh M, Asgharzade S. Evaluation of the Anti-Tumor Effects and Dose-Dependent Mechanisms of Gallic Acid in a Rat Model of Glioblastoma. cmja 2026; 16 (2)
URL: http://cmja.arakmu.ac.ir/article-1-1104-en.html
URL: http://cmja.arakmu.ac.ir/article-1-1104-en.html
Fatemeh Nasr Esfahani1 
, Fatemeh Azizi Farsani2 , Elahe Soltani Fard3 , Mohammad Rahimi Madiseh4 , Samira Asgharzade5
, Fatemeh Azizi Farsani2 , Elahe Soltani Fard3 , Mohammad Rahimi Madiseh4 , Samira Asgharzade5
1- Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
2- Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
3- Medical Plants Research Center, Basic Health Sciences Institute, University of Medical Sciences, Shahrekord, Iran
4- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Iran
5- Shahrekord University of Medical Sciences, Iran.
2- Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
3- Medical Plants Research Center, Basic Health Sciences Institute, University of Medical Sciences, Shahrekord, Iran
4- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Iran
5- Shahrekord University of Medical Sciences, Iran.
Abstract: (45 Views)
Introduction: Glioblastoma (GBM) is the most aggressive primary tumor of the central nervous system, with a poor prognosis. Given the chemo-resistance and rapid tumor recurrence, the development of new therapeutic strategies is essential. Gallic acid (GA) is a natural polyphenol with potent anticancer properties in in vitro studies. This study aimed to evaluate the antitumor effects of GA in a rat animal model of glioblastoma.
Materials and Methods: Rat glioblastoma cell line (C6) was transplanted into the striatum of female Wistar rats using the stereotaxic method. Animals were randomly divided into three groups: 1) control group, 2) group treated with gallic acid (GA) at a dose of 100 mg/kg, and 3) group treated with GA at a dose of 200 mg/kg. The GA was administered intraperitoneally daily for 14 days. After the end of the treatment period, animals were deeply anesthetized, and cardiac perfusion was performed. Then, brain tissue was prepared for histopathological examination by hematoxylin-eosin (H&E) staining, and tumor samples were stored at -80°C for evaluation of VEGF gene expression by Real-time PCR.
Results: Administration of GA resulted in a significant dose-dependent reduction in tumor volume compared to the control group. Histopathological studies showed a decrease in cell density and pseudopalisading necrosis, and a significant inhibition of angiogenesis in the treated groups, and the results of gene expression evaluation showed a dose-dependent decrease in α-VEGF expression in the treated groups.
Conclusion: The findings of this study indicate that GA has a significant antitumor effect in an animal model of glioblastoma. The mechanism of this effect is likely to be through inhibition of angiogenesis (reduction of VEGF) and inhibition of cell proliferation. Gallic acid can be investigated as a promising therapeutic agent for future studies in the field of glioblastoma.
Materials and Methods: Rat glioblastoma cell line (C6) was transplanted into the striatum of female Wistar rats using the stereotaxic method. Animals were randomly divided into three groups: 1) control group, 2) group treated with gallic acid (GA) at a dose of 100 mg/kg, and 3) group treated with GA at a dose of 200 mg/kg. The GA was administered intraperitoneally daily for 14 days. After the end of the treatment period, animals were deeply anesthetized, and cardiac perfusion was performed. Then, brain tissue was prepared for histopathological examination by hematoxylin-eosin (H&E) staining, and tumor samples were stored at -80°C for evaluation of VEGF gene expression by Real-time PCR.
Results: Administration of GA resulted in a significant dose-dependent reduction in tumor volume compared to the control group. Histopathological studies showed a decrease in cell density and pseudopalisading necrosis, and a significant inhibition of angiogenesis in the treated groups, and the results of gene expression evaluation showed a dose-dependent decrease in α-VEGF expression in the treated groups.
Conclusion: The findings of this study indicate that GA has a significant antitumor effect in an animal model of glioblastoma. The mechanism of this effect is likely to be through inhibition of angiogenesis (reduction of VEGF) and inhibition of cell proliferation. Gallic acid can be investigated as a promising therapeutic agent for future studies in the field of glioblastoma.
Type of Study: Research |
Subject:
Pharmacology
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