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Volume 12, Issue 1 (6-2022)
cmja 2022, 12(1): 28-43 |
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Farazandeh S, Taghavizad R, Khalil Moghaddam S. In Vitro Comparison of the Effects of Ailanthus altissima and Salvia Nemorosa Extracts on Pseudomonas aeruginosa, Staphylococcus aureus, and Proteus vulgaris. cmja 2022; 12 (1) :28-43
URL: http://cmja.arakmu.ac.ir/article-1-828-en.html
URL: http://cmja.arakmu.ac.ir/article-1-828-en.html
1- Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran.
2- Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran. , ra_taghavizad@yahoo.com
3- Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran
2- Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran. , ra_taghavizad@yahoo.com
3- Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran
Abstract: (2291 Views)
Objective Some plants have antiseptic properties with no harm to humans or animals. In this study, we aim to assess the effects of Ailanthus altissima and Salvia nemorosa extracts on Pseudomonas aeruginosa, Staphylococcus aureus, and Proteus vulgaris in vitro.
Methods Ailanthus altissima leaves and flowered branch of Salvia nemorosa were collected and dried in shade and powdered. Their aqueous, hydroalcoholic and methanolic extracts were prepared separately from the plants by Maceration method at four concentrations of 20, 30, 40, 50 mg/mL which were used in Mueller-Hinton Agar culture medium by the well diffusion method. Saponin was extracted was using 20% ethanol. The statistical results were analyzed in GraphPad Prism v.7 software.
Results The aqueous extract of Salvia nemorosa was more effective on Pseudomonas aeruginosa (mean non-growth halo diameter = 26±1 mm). The diameter of the non-growth halo with Ailanthus altissima methanolic extract was 17.5±0.5 mm in Pseudomonas aeruginosa and 16.5±0.5 mm in Staphylococcus aureus, and the effect was significant (P<0.0001). Only Ailanthus altissima hydroalcoholic extract had an effect on Proteus vulgaris. High saponin content of two plants increased their effectiveness.
Conclusion Aqueous extract of Salvia nemorosa is recommended to inhibit the growth and proliferation of Pseudomonas aeruginosa.
Methods Ailanthus altissima leaves and flowered branch of Salvia nemorosa were collected and dried in shade and powdered. Their aqueous, hydroalcoholic and methanolic extracts were prepared separately from the plants by Maceration method at four concentrations of 20, 30, 40, 50 mg/mL which were used in Mueller-Hinton Agar culture medium by the well diffusion method. Saponin was extracted was using 20% ethanol. The statistical results were analyzed in GraphPad Prism v.7 software.
Results The aqueous extract of Salvia nemorosa was more effective on Pseudomonas aeruginosa (mean non-growth halo diameter = 26±1 mm). The diameter of the non-growth halo with Ailanthus altissima methanolic extract was 17.5±0.5 mm in Pseudomonas aeruginosa and 16.5±0.5 mm in Staphylococcus aureus, and the effect was significant (P<0.0001). Only Ailanthus altissima hydroalcoholic extract had an effect on Proteus vulgaris. High saponin content of two plants increased their effectiveness.
Conclusion Aqueous extract of Salvia nemorosa is recommended to inhibit the growth and proliferation of Pseudomonas aeruginosa.
Type of Study: Research |
Subject:
Medicinal Plants
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