Enter your email address
Submit
Write your message
Volume 13, Issue 4 (1-2024)
cmja 2024, 13(4): 44-53 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Firoozi M, Barari A, Abbassi Daloii A, Abednatanzi H. The Effect of Interval Training and Urtica Dioica Hydroalcoholic Extract on some Growth and Immune Factors in C57 Rats with Melanoma Cancer. cmja 2024; 13 (4) :44-53
URL: http://cmja.arakmu.ac.ir/article-1-962-en.html
URL: http://cmja.arakmu.ac.ir/article-1-962-en.html
1- Ph.D Student, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,alireza54.barari@gmail.com
3- Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
4- Assistance Professor, Department of Physical Education and sport science, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
2- Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,
3- Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
4- Assistance Professor, Department of Physical Education and sport science, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
Abstract: (892 Views)
Introduction: Introduction: Exercise interventions combined with medicinal plants are effective in preventing or reducing cancer symptoms and side effects caused by cancer treatment. The aim of this study was to investigate the effect of interval training and consumption of Urtica dioica hydroalcoholic extract on selecteted factors of immune and growth in C57BL/6 rats with melanoma cancer.
Methods: In this experimental study, 20 C57BL/6 rats were randomly divided into four groups include control (cancer), Urtica dioica (cancer), and Urtica dioica - interval training (cancer). Experimental groups received nettle extract of 30 mg daily per kilogram of body weight. The interval exercise program included running on a treadmill 60 minutes at a speed of 28 meters per minute, five days a week for six weeks. Levels of nucleotide exchange factor (Apg-2), heat shock protein 70 (HSP70), cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), programmed death protein-1 (PD-1), insulin-like growth factor-binding protein 2 (IGFBP2) of rats was measured by Real Time PCR method. The data were analyzed and analyzed by One -way variance analysis and the Toky's test at the P<0.05.
Results: interval training led to significant increase in gene expression of APG2, CTLA4 and decrease in IGFBP2 (P<0.001). Urtica dioica extract led to significant decrease in IGFBP2 (P<0.010). interval training with Urtica dioica extract led to significant increase in gene expression of APG2 and decrease in IGFBP2 compared to the Urtica dioica group (P<0.001). There was no significant difference between the mean of PD-1 gene expression in different groups (P=0.158).
Conclusions: According to the results, interval training with Urtica dioica hydroalcoholic consumption has more advantages in regulating gene expression of some growth and immune factors in melanoma cancer than interval training and Urtica dioica consumption alone.
Methods: In this experimental study, 20 C57BL/6 rats were randomly divided into four groups include control (cancer), Urtica dioica (cancer), and Urtica dioica - interval training (cancer). Experimental groups received nettle extract of 30 mg daily per kilogram of body weight. The interval exercise program included running on a treadmill 60 minutes at a speed of 28 meters per minute, five days a week for six weeks. Levels of nucleotide exchange factor (Apg-2), heat shock protein 70 (HSP70), cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), programmed death protein-1 (PD-1), insulin-like growth factor-binding protein 2 (IGFBP2) of rats was measured by Real Time PCR method. The data were analyzed and analyzed by One -way variance analysis and the Toky's test at the P<0.05.
Results: interval training led to significant increase in gene expression of APG2, CTLA4 and decrease in IGFBP2 (P<0.001). Urtica dioica extract led to significant decrease in IGFBP2 (P<0.010). interval training with Urtica dioica extract led to significant increase in gene expression of APG2 and decrease in IGFBP2 compared to the Urtica dioica group (P<0.001). There was no significant difference between the mean of PD-1 gene expression in different groups (P=0.158).
Conclusions: According to the results, interval training with Urtica dioica hydroalcoholic consumption has more advantages in regulating gene expression of some growth and immune factors in melanoma cancer than interval training and Urtica dioica consumption alone.
Type of Study: Research |
Subject:
Physiology
References
1. Huang P, Chang S, Jiang X, Su J, Dong C, Liu X. RNA interference targeting CD147 inhibits the proliferation, invasiveness, and metastatic activity of thyroid carcinoma cells by down-regulating glycolysis. Int J Clin Exp Pathol. 2015;8(1):309-18.
2. Kim T, Yoon S, Shin DE, Lee SC, Oh J, Lee SY, et al. Incidence and Survival Rates of Cutaneous Melanoma in South Korea Using Nationwide Health Insurance Claims Data. Cancer research and treatment. 2022;54(3):937-49. [DOI:10.4143/crt.2021.871] [PMID] []
3. Ilham S, Willis C, Kim K, Chung KC, Wood BM, Tan MS, et al. Cancer incidence in immunocompromised patients: a single-center cohort study. BMC cancer. 2023;23(1):33. [DOI:10.1186/s12885-022-10497-4] [PMID] []
4. Han SH, Yoo SG, Do Han K, La Y, Kwon DE, Lee KH. The Incidence and Effect of Cytomegalovirus Disease on Mortality in Transplant Recipients and General Population: Real-world Nationwide Cohort Data. International journal of medical sciences. 2021;18(14):3333-41. [DOI:10.7150/ijms.62621] [PMID] []
5. Zhang B, Hong CQ, Luo YH, Wei LF, Luo Y, Peng YH, et al. Prognostic value of IGFBP2 in various cancers: a systematic review and meta-analysis. Cancer medicine. 2022;11(16):3035-47. [DOI:10.1002/cam4.4680] [PMID] []
6. Wei LF, Weng XF, Huang XC, Peng YH, Guo HP, Xu YW. IGFBP2 in cancer: Pathological role and clinical significance (Review). Oncology reports. 2021;45(2):427-38. [DOI:10.3892/or.2020.7892] [PMID]
7. Pickard A, McCance DJ. IGF-Binding Protein 2 - Oncogene or Tumor Suppressor? Frontiers in endocrinology. 2015;6:25. [DOI:10.3389/fendo.2015.00025] [PMID] []
8. Jayaraj GG, Hipp MS, Hartl FU. Functional Modules of the Proteostasis Network. Cold Spring Harbor perspectives in biology. 2020;12(1). [DOI:10.1101/cshperspect.a033951] [PMID] []
9. Kimura A, Ogata K, Altan B, Yokobori T, Ide M, Mochiki E, et al. Nuclear heat shock protein 110 expression is associated with poor prognosis and chemotherapy resistance in gastric cancer. Oncotarget. 2016;7(14):18415-23. [DOI:10.18632/oncotarget.7821] [PMID] []
10. Fife BT, Bluestone JA. Control of peripheral T-cell tolerance and autoimmunity via the CTLA-4 and PD-1 pathways. Immunological reviews. 2008;224:166-82. [DOI:10.1111/j.1600-065X.2008.00662.x] [PMID]
11. Keir ME, Butte MJ, Freeman GJ, Sharpe AH. PD-1 and its ligands in tolerance and immunity. Annual review of immunology. 2008;26:677-704. [DOI:10.1146/annurev.immunol.26.021607.090331] [PMID] []
12. Friedenreich CM, Stone CR, Cheung WY, Hayes SC. Physical Activity and Mortality in Cancer Survivors: A Systematic Review and Meta-Analysis. JNCI cancer spectrum. 2020;4(1):pkz080. [DOI:10.1093/jncics/pkz080] [PMID] []
13. Yang L, Morielli AR, Heer E, Kirkham AA, Cheung WY, Usmani N, et al. Effects of Exercise on Cancer Treatment Efficacy: A Systematic Review of Preclinical and Clinical Studies. Cancer research. 2021;81(19):4889-95. [DOI:10.1158/0008-5472.CAN-21-1258] [PMID] []
14. Crosby BJ, Lopez P, Galvao DA, Newton RU, Taaffe DR, Meniawy TM, et al. Associations of Physical Activity and Exercise with Health-related Outcomes in Patients with Melanoma During and After Treatment: A Systematic Review. Integrative cancer therapies. 2021;20:15347354211040757. [DOI:10.1177/15347354211040757] [PMID] []
15. Bay ML, Unterrainer N, Stagaard R, Pedersen KS, Schauer T, Staffeldt MM, et al. Voluntary wheel running can lead to modulation of immune checkpoint molecule expression. Acta oncologica. 2020;59(12):1447-54. [DOI:10.1080/0284186X.2020.1817550] [PMID]
16. Jafari A, Arazi H, Ghadian A, Hesrak K. The Impact of Combined (aerobic-resistance) Training on Serum Levels of IGF-I and IGFBP-3 in Men with Prostate Cancer. J Adv Med Biomed Res. 2019;27(122):35-41. [DOI:10.30699/jambs.27.122.35]
17. Esposito S, Bianco A, Russo R, Di Maro A, Isernia C, Pedone PV. Therapeutic Perspectives of Molecules from Urtica dioica Extracts for Cancer Treatment. Molecules. 2019;24(15). [DOI:10.3390/molecules24152753] [PMID] []
18. Catoni CH, Schaefer HM, Peters A. Fruit for health: the effect of flavonoids on humoral immune response and food se- lection in a frugivorous bird. Funct Ecol. 2008;22:644- 54. [DOI:10.1111/j.1365-2435.2008.01400.x]
19. Avci G, Kupeli E, Eryavuz A, Yesilada E, Kucukkurt I. Antihypercholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine. Journal of ethnopharmacology. 2006;107(3):418-23. [DOI:10.1016/j.jep.2006.03.032] [PMID]
20. D'Abrosca B, Ciaramella V, Graziani V, Papaccio F, Della Corte CM, Potenza N, et al. Urtica dioica L. inhibits proliferation and enhances cisplatin cytotoxicity in NSCLC cells via Endoplasmic Reticulum-stress mediated apoptosis. Scientific reports. 2019;9(1):4986. [DOI:10.1038/s41598-019-41372-1] [PMID] []
21. Kocasli S, Demircan Z. Herbal Product Use by the Cancer Patients in Both the Pre and Post Surgery Periods and during Chemotherapy. African journal of traditional, complementary, and alternative medicines : AJTCAM. 2017;14(2):325-33. [DOI:10.21010/ajtcam.v14i2.34] [PMID] []
22. Amjadi F, Javanmard SH, Zarkesh-Esfahani H, Khazaei M, Narimani M. Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production. Journal of experimental & clinical cancer research : CR. 2011;30(1):21. [DOI:10.1186/1756-9966-30-21] [PMID] []
23. Hodroj MH, Al Bast NAH, Taleb RI, Borjac J, Rizk S. Nettle Tea Inhibits Growth of Acute Myeloid Leukemia Cells In Vitro by Promoting Apoptosis. Nutrients. 2020;12(9). [DOI:10.3390/nu12092629] [PMID] []
24. Zolghadri V, Barari A, Abbasi daloii A, Abed natanzi H. The effect of consumption nettle extract and aerobic training on MMP2 and FGF2 gene expression in mice with melanoma. IJCA. 2021;2(3):11-20.
25. Wennerberg E, Lhuillier C, Rybstein MD, Dannenberg K, Rudqvist NP, Koelwyn GJ, et al. Exercise reduces immune suppression and breast cancer progression in a preclinical model. Oncotarget. 2020;11(4):452-61. [DOI:10.18632/oncotarget.27464] [PMID] []
26. Ogata T, Oishi Y, Higashida K, Higuchi M, Muraoka I. Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. American journal of physiology Regulatory, integrative and comparative physiology. 2009;296(5):R1557-63. [DOI:10.1152/ajpregu.90911.2008] [PMID]
27. Lang BJ, Guerrero-Gimenez ME, Prince TL, Ackerman A, Bonorino C, Calderwood SK. Heat Shock Proteins Are Essential Components in Transformation and Tumor Progression: Cancer Cell Intrinsic Pathways and Beyond. International journal of molecular sciences. 2019;20(18). [DOI:10.3390/ijms20184507] [PMID] []
28. Pedersen L, Idorn M, Olofsson GH, Lauenborg B, Nookaew I, Hansen RH, et al. Voluntary Running Suppresses Tumor Growth through Epinephrine- and IL-6-Dependent NK Cell Mobilization and Redistribution. Cell metabolism. 2016;23(3):554-62. [DOI:10.1016/j.cmet.2016.01.011] [PMID]
29. Tehrani zadeh TFM, Darzabi T, Hejazi K, Nasery H. Effects of Aerobic Training on HSP70, Liver Enzymes and Insulin Resistance index in Elderly Women with Non-Alcoholic Fatty Liver in Bojnourd, Iran. Nurs Vulnerable J. 2018;4(13):1-18.
30. Abdollahzadeh Soreshjani S, Ashrafizadeh M. Effects of exercise on testosterone level, heat shock protein, and fertility potential. Rev Clinic Med. 2018;5(4):141-5.
31. Salehian O, Soori R, Mohammadhassan Z, Ravasi AA. The Effect of Interval Training on Tumor Volume in Mice with Breast Cancer. Knowledge Health. 2014;8(4):144-9.
32. Ivy JL, Zderic TW, Fogt DL. Prevention and treatment on non-insulindependent diabetes mellitus. Exerc Sport Sci Rev. 1999;27(40):1-35. [DOI:10.1249/00003677-199900270-00003]
33. Meneses-Echávez JF, Ramírez-Vélez R, Gonzalez-Jimenez E, Sanchez-Perez MJ, Molina-Montes E. Physical activity and Insulin-like growth factors in breast cancer survivors: A metaanalysis. Eur J Surg Oncol. 2014;40(11):S65. [DOI:10.1016/j.ejso.2014.08.141]
34. Nindl BC, Kraemer WJ, Marx JO, Arciero PJ, Dohi K, Kellogg MD, et al. Overnight responses of the circulating IGF-I system after acute, heavy-resistance exercise. Journal of applied physiology. 2001;90(4):1319-26. [DOI:10.1152/jappl.2001.90.4.1319] [PMID]
35. Chan DV, Gibson HM, Aufiero BM, Wilson AJ, Hafner MS, Mi QS, et al. Differential CTLA-4 expression in human CD4+ versus CD8+ T cells is associated with increased NFAT1 and inhibition of CD4+ proliferation. Genes and immunity. 2014;15(1):25-32. [DOI:10.1038/gene.2013.57] [PMID] []
36. Dos Santos CMM, Diniz VLS, Bachi ALL, Dos Santos de Oliveira LC, Ghazal T, Passos MEP, et al. Moderate physical exercise improves lymphocyte function in melanoma-bearing mice on a high-fat diet. Nutrition & metabolism. 2019;16:63. [DOI:10.1186/s12986-019-0394-z] [PMID] []
37. Idorn M, Hojman P. Exercise-Dependent Regulation of NK Cells in Cancer Protection. Trends in molecular medicine. 2016;22(7):565-77. [DOI:10.1016/j.molmed.2016.05.007] [PMID]
38. Taube JM, Anders RA, Young GD, Xu H, Sharma R, McMiller TL, et al. Colocalization of inflammatory response with B7-h1 expression in human melanocytic lesions supports an adaptive resistance mechanism of immune escape. Science translational medicine. 2012;4(127):127ra37. [DOI:10.1126/scitranslmed.3003689] [PMID] []
39. Sun C, Mezzadra R, Schumacher TN. Regulation and Function of the PD-L1 Checkpoint. Immunity. 2018;48(3):434-52. [DOI:10.1016/j.immuni.2018.03.014] [PMID] []
40. Wang RJ, Li DF, Bourne S, Wang RJ, Li DF. Can 2000 years of herbal medicine history help us solve problems in the year 2000? Presented at the Alltech's 14th Annual Symposium on Biotechnology in the Feed Industry, Nicholasville, KY, USA,1998. 273-92 p.
41. Konrad L, Muller HH, Lenz C, Laubinger H, Aumuller G, Lichius JJ. Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Planta medica. 2000;66(1):44-7. [DOI:10.1055/s-2000-11117] [PMID]
42. Ding Z, Dai Y, Hao H, Pan R, Yao X, Wang Z. Anti-inflammatory e ects of scopoletin and underlying mechanisms. Pharm Biol. 2008;46:854-60. [DOI:10.1080/13880200802367155]
43. Liu Y, Steinacker JM. Changes in skeletal muscle heat shock proteins: Pathologcal significance. Front Biosci. 2001;6:12-25.
https://doi.org/10.2741/A590 [DOI:10.2741/Liu]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Contact Information
Complementary Medicine Journal
Arak University of Medical Sciences, Sardasht, Arak, Iran.
Journal Tel: +98 86 34173524
Email: journalcmjaarakmu.ac.ir
