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Volume 14, Issue 3 (11-2024)
cmja 2024, 14(3): 57-66 |
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Sharifi H, Etamad Z, Azizbeigi K, Farzanegi P. Investigating the effects of aerobic training and stem cell therapy on the expression of TNFα gene and MMP13 protein in knee cartilage tissue in male rats of osteoarthritis model. cmja 2024; 14 (3) :57-66
URL: http://cmja.arakmu.ac.ir/article-1-997-en.html
URL: http://cmja.arakmu.ac.ir/article-1-997-en.html
1- PhD Candidate in Exercise Physiology, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
2- Assistant Professor in Exercise Physiology, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. ,zetemad2002@yahoo.com
3- Associate Professor in Exercise Physiology, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
4- Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
2- Assistant Professor in Exercise Physiology, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. ,
3- Associate Professor in Exercise Physiology, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
4- Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
Abstract: (607 Views)
Background and purpose: Exercise and stem cell therapy have promising effects on the management of osteoarthritis. Tumor necrosis factor-α (TNFα) and matrix metalloproteinase-13 (MMP13) are two important factors related to joint inflammation and cartilage destruction. The present study examines the effects of aerobic training and stem cell injection on the expression of the TNFα gene and MMP13 protein in knee cartilage tissue in male rats with osteoarthritis.
Materials and methods: In the present experimental study, 25 rats were randomly divided into healthy, osteoarthritis, osteoarthritis-exercise, osteoarthritis-stem cell, and osteoarthritis-stem cell-exercise groups and received interventions based on the group name. Endurance training was performed for eight weeks (5 sessions/week, 30-50 minutes/session, and at a speed of 16 meters/minute) on a non-inclined treadmill. Mesenchymal stem cells (1x106 cells/kg) were injected once to the respective groups. Forty-eight hours after the last training session, cartilage tissue was removed and sent to the laboratory. One-way analysis of variance and Tukey's post hoc test were used to determine the difference between groups. All calculations were done with SPSS software, and the significance level was considered as P≤0.05.
Results: TNFα gene expression was higher in the osteoarthritis group than in the healthy group (P=0.027). There was no other significant difference in TNFα gene expression between the groups. MMP13 protein expression was higher in osteoarthritis (P=0.001), osteoarthritis-exercise (P=0.003), osteoarthritis-stem cell (P=0.011), and osteoarthritis-stem cell-exercise (P=0.001) groups than in the healthy group. MMP13 protein expression in osteoarthritis-exercise (P=0.001), osteoarthritis-stem cell (P=0.001), and osteoarthritis-stem cell-exercise (P=0.001) groups was lower than the osteoarthritis group. There was no other significant difference in the expression of MMP13 protein between the groups.
Conclusion: Endurance training and stem cell therapy have a positive role in modulating MMP13 protein expression, which supports the potential of these two interventions to improve osteoarthritis.
Materials and methods: In the present experimental study, 25 rats were randomly divided into healthy, osteoarthritis, osteoarthritis-exercise, osteoarthritis-stem cell, and osteoarthritis-stem cell-exercise groups and received interventions based on the group name. Endurance training was performed for eight weeks (5 sessions/week, 30-50 minutes/session, and at a speed of 16 meters/minute) on a non-inclined treadmill. Mesenchymal stem cells (1x106 cells/kg) were injected once to the respective groups. Forty-eight hours after the last training session, cartilage tissue was removed and sent to the laboratory. One-way analysis of variance and Tukey's post hoc test were used to determine the difference between groups. All calculations were done with SPSS software, and the significance level was considered as P≤0.05.
Results: TNFα gene expression was higher in the osteoarthritis group than in the healthy group (P=0.027). There was no other significant difference in TNFα gene expression between the groups. MMP13 protein expression was higher in osteoarthritis (P=0.001), osteoarthritis-exercise (P=0.003), osteoarthritis-stem cell (P=0.011), and osteoarthritis-stem cell-exercise (P=0.001) groups than in the healthy group. MMP13 protein expression in osteoarthritis-exercise (P=0.001), osteoarthritis-stem cell (P=0.001), and osteoarthritis-stem cell-exercise (P=0.001) groups was lower than the osteoarthritis group. There was no other significant difference in the expression of MMP13 protein between the groups.
Conclusion: Endurance training and stem cell therapy have a positive role in modulating MMP13 protein expression, which supports the potential of these two interventions to improve osteoarthritis.
Keywords: Endurance training, Exercise, Mesenchymal stem cells, Stem cell injection, Therapeutic interventions.
Type of Study: Research |
Subject:
Physiology
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