INTRODUCTION
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and the main cause of hyperandrogenism and multiple anovulation, which leads to infertility. Evidence suggests that obese women are more susceptible to developing PCOS, with approximately 60% of those with PCOS being obese. Recently, AMH has been shown to be a biomarker of oocyte reserve for early detection of ovarian follicular loss and the onset of menopause. Currently, lifestyle modifications, including physical activity and diet, are recommended as first-line and initial treatment options. High-intensity interval training (HIIT) is one of the exercises that has attracted the attention of many researchers and different people in recent years. Regarding the nutritional strategy for combating and treating PCOS, the Dietary Approaches to Stop Hypertension (DASH) diet is a low-glycemic, low-calorie diet that was initially considered for regulating blood pressure. Therefore, the present study aimed to determine the effect of HIIT and the DASH diet on biomarkers of oocyte reserve and insulin resistance in obese women with PCOS.

METHODS
The present study followed the semi-experimental and applied research protocols, which was conducted as a pre-test and post-test in a 2x2 design. The present study is a quasi-experimental and applied study that was conducted as a pre-test and post-test in a 2×2 design. The statistical population of the study included obese women (BMI≥30 kg/m²) with PCOS aged 18 to 40 years (reproductive age) in Ardabil city. A total of 40 obese women with PCOS were randomly divided into four groups: control, DASH diet, interval training, and interval training+DASH diet. The HIIT was performed for eight weeks (three sessions in a week are performed in supervised forms, and two sessions in a week are performed in unsupervised forms). Diet groups followed the DASH diet for eight weeks. Anti-Müllerian (AMH) and homeostasis model assessment of insulin resistance (HOMA-IR) index were measured before and 48 h after the last training session and healthy diet. The analysis of data was performed using the factor analysis of variance test at a significance level of P<0.05.

RESULTS
According to the results of the Shapiro-Wilk test, the collected data had a normal distribution, and there was no significant difference between the study groups in the indicators considered before the intervention and at baseline. The effect of HIIT exercise (F=0.27; P=0.15) and DASH diet (F=0.16; P=0.69) alone, and the effect of exercise×diet (F=1.07; P=0.33) on AMH level is not significant. However, the amount of this hormone decreased by approximately 1.5% in the HIIT group, 3.6% in the DASH diet group, and 7.8% in the exercise+diet group. However, the HIIT exercise (F=26.7; P=0.001) and DASH diet (F=100.1; P=0.0001) alone and exercise×diet (F=45.8; P=0.001) significantly reduced insulin resistance.
Table 1. Effect of interventions on AMH hormone and HOMA-IR index