Introduction: The perpose of this research was the investigation of 8-week  regulary aerobic intensity exercise effects and Omega-3 supplementation on Total level antioxidant capacity and hydrogen peroxide in young untrained men.

Method: This research was a semi-experimental method with repeated measures. Among the volunteers, 20 subjects after obtaining informed consent participated in the study in two groups of 8 week omega-3 Supplements with moderate exercise (10 subjects) and only moderate exercise (10 subjects). Blood sample were taken in Basal state, after 4 and 8 weeks in both groups for evaluation of Total antioxidant capacity and Hydrogen peroxide levels. Also, Anova and Bonferroni statically analyze test whit SPSS 22 was used for intra group change markers and between compared.

Results: The results showed that Total antioxidant capacity significantly increased in both groups after 4 (omega3+exercise p=0.003, exercise p=0.004) & 8 weeks (both groups p=0.001). But Supplement omega-3+exercise group has higher levels of TAC in compared with exercise group (p=0.001). Hydrogen Peroxide significantly decreased in both groups after 4 & 8 weeks (in both groups all stage p=0.001), but Supplement Omega-3+exercise group has lower levels of H2O2 in compared with exercise group after 8 week (p=0.001).

Conclusion: at the end it was concleded that using omega-3 during the eight weeks moderate aerobic exercise, possibly further increased the total Antioxidant Capacity and  the hydrogen peroxide significantly  reduced.