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Volume 14, Issue 1 (4-2024)
cmja 2024, 14(1): 38-44 |
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Mousavi H, Badri H, Najafi E, Parastar S, Nazari S. Comparing the Anti-candidal Effect of Egg White (Machine and Local) with Amphotericin B in Vitro. cmja 2024; 14 (1) :38-44
URL: http://cmja.arakmu.ac.ir/article-1-971-en.html
URL: http://cmja.arakmu.ac.ir/article-1-971-en.html
1- Student Research Committee, Khalkhal University of Medical Sciences, Khalkhal, Iran
2- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran
3- Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran & Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran
4- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran ,Shahramnazari73@yahoo.com
2- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran
3- Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran & Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran
4- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran ,
Abstract: (248 Views)
Introduction: The increase in the resistance of fungal species and the high side effects of antifungal drugs have led researchers to study and find new treatment methods against fungi. This study evaluated the anti-candidacy effects of local and artificial egg whites and compared them with amphotericin B.
Materials and Methods: This study was conducted experimentally in May 2023 in the Microbiology Laboratory of Khalkhal Faculty of Medical Sciences. This research tested the antifungal effects of local and machine chicken egg whites and amphotericin B using the micro broth dilution and zone of inhibition method. In this experiment, Candida albicans species were placed in the vicinity of different concentrations of substances, and the minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) of these substances on C. albicans were determined according to the guidelines of the Clinical and Laboratory Standards Institute.
This study has been registered with the ethical code IR. IR.KHALUMS.REC.1401.019 in the Ethics Committee of Khalkhal Faculty of Medical Sciences.
Results: Based on the results of the tests, the MIC of local and machine chicken egg whites on C. albicans was 25%, and their MFC was 50%. However, in these concentrations, no growth was observed in the presence of amphotericin B. Moreover, the MIC and MFC values of 25 µl of amphotericin B with 75 µl of local and machine egg whites were 3.125% and 6.25%, respectively. The zone of inhibition for C. albicans in the vicinity of local and machine chicken egg white and amphotericin B was 12, 11, and 16 mm, respectively.
Conclusion: Local and machine chicken egg whites have antifungal properties against C. albicans.
Materials and Methods: This study was conducted experimentally in May 2023 in the Microbiology Laboratory of Khalkhal Faculty of Medical Sciences. This research tested the antifungal effects of local and machine chicken egg whites and amphotericin B using the micro broth dilution and zone of inhibition method. In this experiment, Candida albicans species were placed in the vicinity of different concentrations of substances, and the minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) of these substances on C. albicans were determined according to the guidelines of the Clinical and Laboratory Standards Institute.
This study has been registered with the ethical code IR. IR.KHALUMS.REC.1401.019 in the Ethics Committee of Khalkhal Faculty of Medical Sciences.
Results: Based on the results of the tests, the MIC of local and machine chicken egg whites on C. albicans was 25%, and their MFC was 50%. However, in these concentrations, no growth was observed in the presence of amphotericin B. Moreover, the MIC and MFC values of 25 µl of amphotericin B with 75 µl of local and machine egg whites were 3.125% and 6.25%, respectively. The zone of inhibition for C. albicans in the vicinity of local and machine chicken egg white and amphotericin B was 12, 11, and 16 mm, respectively.
Conclusion: Local and machine chicken egg whites have antifungal properties against C. albicans.
Keywords: : Local and machine chicken egg white, Candida albicans, amphotericin B, antifungal effect
Type of Study: Research |
Subject:
Traditional medicine
References
1. Odds FC. Candida infections: an overview. Crit Rev Microbiol. 1987;15(1):1-5. [doi:10.3109/10408418709104444] [pmid:3319417]
2. Lockhart SR. Current epidemiology of Candida infection. Clinical Microbiology Newsletter. 2014;36(17):131-6. [doi:10.1016/j.clinmicnews.2014.08.001]
3. Bouchelaghem S. Propolis characterization and antimicrobial activities against Staphylococcus aureus and Candida albicans: A review. Saudi J Biol Sci. 2022;29(4):1936-1946. [doi:10.1016/j.sjbs.2021.11.063] [pmid:35531223]
4. Boroujeni ZB, Shamsaei S, Yarahmadi M, Getso MI, Khorashad AS, Haghighi L, et al. Distribution of invasive fungal infections: Molecular epidemiology, etiology, clinical conditions, diagnosis and risk factors: A 3-year experience with 490 patients under intensive care. Microb Pathog. 2021;152:104616. [doi:10.1016/j.micpath.2020.104616] [pmid:33212195]
5. Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont. 2011;20(4):251-60. [doi:10.1111/j.1532-849X.2011.00698.x] [pmid:21463383]
6. Alonso GC, Klein MI, Jordão CC, Carmello JC, Pavarina AC. Gene expression of Candida albicans strains isolates from patients with denture stomatitis submitted to treatments with photodynamic therapy and nystatin. Photodiagnosis Photodyn Ther. 2021;35:102292. [doi:10.1016/j.pdpdt.2021.102292] [pmid:33857598]
7. Taher JM, Raheem NN. Role of purified beta-glucanase from lactobacillus acidophilus in disruption of biofilm formation by candida spp. causing of denture stomatitis. Materials Today: Proceedings. 2022;60(22):1507-12. [doi:10.1016/j.matpr.2021.12.021]
8. Yarborough A, Cooper L, Duqum I, Mendonça G, McGraw K, Stoner L. Evidence regarding the treatment of denture stomatitis. J Prosthodont. 2016;25(4):288-301. [doi:10.1111/jopr.12454] [pmid:27062660]
9. Lombardi T, Budtz-Jørgensen E. Treatment of denture-induced stomatitis: a review. Eur J Prosthodont Restor Dent. 1993;2(1):17-22. pmid: 8180613 [pmid:8180613]
10. Rathee M, Maqbul Alam DPJ, Shetye A. Prosthodontic management of a completely edentulous diabetic patient with Mucormycosis- a case report. From the Editor's Desk. 2022.
11. Budtz‐Jörgensen E, LöE H. Chlorhexidine as a denture disinfectant in the treatment of denture stomatitis. Scand J Dent Res. 1972;80(6):457-64. [doi:10.1111/j.1600-0722.1972.tb00314.x] [pmid:4575037]
12. Petrović M, Kostić M, Kostić M, Krunić N, Igić M, Pešić Z, et al. Therapeutic alternatives of natural compounds in treatment of Candida-associated denture stomatitis. Acta Medica Medianae. 2014;53(1):73-9. [doi:10.5633/amm.2014.0113]
13. Nasrollahi A, Pourshamsian K, Mansourkiaee P. Antifungal activity of silver nanoparticles on some of fungi. Int J Nano Dim. 2011:1(3):233-239. [doi:10.7508/ijnd.2010.03.007]
14. Jalili-Firoozinezhad S, Filippi M, Mohabatpour F, Letourneur D, Scherberich A. Chicken egg white: Hatching of a new old biomaterial. Materials Today. 2020;40:193-214. [doi:10.1016/j.mattod.2020.05.022]
15. Legros J, Jan S, Bonnassie S, Gautier M, Croguennec T, Pezennec S, et al. The role of ovotransferrin in egg-white antimicrobial activity: A review. Foods. 2021;10(4):823. [doi:10.3390/foods10040823] [pmid:33920211]
16. Feeney RE, Nagy DA. The antibacterial activity of the egg white protein conalbumin. J Bacteriol. 1952;64(5):629-43. [doi:10.1128/jb.64.5.629-643.1952] [pmid:12999693]
17. Carrillo W, García-Ruiz A, Recio I, Moreno-Arribas M. Antibacterial activity of hen egg white lysozyme modified by heat and enzymatic treatments against oenological lactic acid bacteria and acetic acid bacteria. J Food Prot. 2014;77(10):1732-9. [doi:10.4315/0362-028X.JFP-14-009] [pmid:25285490]
18. Wellman-Labadie O, Picman J, Hincke M. Comparative antibacterial activity of avian egg white protein extracts. Br Poult Sci. 2008;49(2):125-32. [doi:10.1080/00071660801938825] [pmid:18409086]
19. Huang X, Zhou X, Jia B, Li N, Jia J, He M, et al. Transcriptional sequencing uncovers survival mechanisms of Salmonella enterica serovar Enteritidis in antibacterial egg white. mSphere. 2019;4(1):e00700-18. [doi:10.1128/mSphere.00700-18] [pmid:30760616]
20. Ma B, Guo Y, Fu X, Jin Y. Identification and antimicrobial mechanisms of a novel peptide derived from egg white ovotransferrin hydrolysates. Lwt. 2020;131:109720. [doi:10.1016/j.lwt.2020.109720]
21. Brand J, Dachmann E, Pichler M, Lotz S, Kulozik U. A novel approach for lysozyme and ovotransferrin fractionation from egg white by radial flow membrane adsorption chromatography: Impact of product and process variables. Separation and Purification Technology. 2016;161:44-52. [doi:10.1016/j.seppur.2016.01.032]
22. Salimpour Abkenar S, Mohammad Ali Malek R. Preparation, characterization, and antimicrobial property of cotton cellulose fabric grafted with poly (propylene imine) dendrimer. Cellulose. 2012;19:1701-14. [doi:10.1007/s10570-012-9744-y]
23. Strydom SJ, Rose WE, Otto DP, Liebenberg W, De Villiers MM. Poly (amidoamine) dendrimer-mediated synthesis and stabilization of silver sulfonamide nanoparticles with increased antibacterial activity. Nanomedicine. 2013;9(1):85-93. [doi:10.1016/j.nano.2012.03.006] [pmid:22470054]
24. Gholami M, Mohammadi R, Arzanlou M, Akbari Dourbash F, Kouhsari E, Majidi G, et al. In vitro antibacterial activity of poly (amidoamine)-G7 dendrimer. BMC Infect Dis. 2017;17(1):395. [doi:10.1186/s12879-017-2513-7] [pmid:28583153]
25. Cantón E, Espinel-Ingroff A, Pemán J. Trends in antifungal susceptibility testing using CLSI reference and commercial methods. Expert Rev Anti Infect Ther. 2009;7(1):107-19. [doi:10.1586/14787210.7.1.107] [pmid:19622060]
26. Kolaczkowska A, Kolaczkowski M, Sokolowska A, Miecznikowska H, Kubiak A, Rolka K, et al. The antifungal properties of chicken egg cystatin against Candida yeast isolates showing different levels of azole resistance. Mycoses. 2010;53(4):314-20. [doi:10.1111/j.1439-0507.2009.01722.x] [pmid:19549107]
27. Shimazaki Y, Takahashi A. Antibacterial activity of lysozyme-binding proteins from chicken egg white. J Microbiol Methods. 2018;154:19-24. [doi:10.1016/j.mimet.2018.10.001] [pmid:30291881]
28. Moreno LSS, Junior HVN, da Silva AR, do Nascimento FBSA, da Silva CR, de Andrade Neto JB, et al. Arginine-phenylalanine and arginine-tryptophan-based surfactants as new biocompatible antifungal agents and their synergistic effect with Amphotericin B against fluconazole-resistant Candida strains. Colloids Surf B Biointerfaces. 2021;207:112017. [doi:10.1016/j.colsurfb.2021.112017] [pmid:34391169]
29. Azevedo M, Ramalho P, Silva A, Teixeira-Santos R, Pina-Vaz C, Rodrigues A. Polyethyleneimine and polyethyleneimine-based nanoparticles: novel bacterial and yeast biofilm inhibitors. J Med Microbiol. 2014;63(9):1167-1173. [doi:10.1099/jmm.0.069609-0] [pmid:24913563]
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