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Volume 14, Issue 1 (4-2024)
cmja 2024, 14(1): 38-44 |
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Mousavi H, Badri H, Najafi E, Parastar S, Nazari S. Comparing the Anti-candidal Effect of Egg White (Machine and Local) with Amphotericin B in Vitro. cmja 2024; 14 (1) :38-44
URL: http://cmja.arakmu.ac.ir/article-1-971-en.html
URL: http://cmja.arakmu.ac.ir/article-1-971-en.html
1- Student Research Committee, Khalkhal University of Medical Sciences, Khalkhal, Iran
2- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran
3- Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran & Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran
4- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran ,Shahramnazari73@yahoo.com
2- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran
3- Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran & Department of Public Health, Khalkhal University of Medical Sciences, Khalkhal, Iran
4- Department of Environment Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran ,
Abstract: (169 Views)
Introduction: The increase in the resistance of fungal species and the high side effects of antifungal drugs have led researchers to study and find new treatment methods against fungi. This study evaluated the anti-candidacy effects of local and artificial egg whites and compared them with amphotericin B.
Materials and Methods: This study was conducted experimentally in May 2023 in the Microbiology Laboratory of Khalkhal Faculty of Medical Sciences. This research tested the antifungal effects of local and machine chicken egg whites and amphotericin B using the micro broth dilution and zone of inhibition method. In this experiment, Candida albicans species were placed in the vicinity of different concentrations of substances, and the minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) of these substances on C. albicans were determined according to the guidelines of the Clinical and Laboratory Standards Institute.
This study has been registered with the ethical code IR. IR.KHALUMS.REC.1401.019 in the Ethics Committee of Khalkhal Faculty of Medical Sciences.
Results: Based on the results of the tests, the MIC of local and machine chicken egg whites on C. albicans was 25%, and their MFC was 50%. However, in these concentrations, no growth was observed in the presence of amphotericin B. Moreover, the MIC and MFC values of 25 µl of amphotericin B with 75 µl of local and machine egg whites were 3.125% and 6.25%, respectively. The zone of inhibition for C. albicans in the vicinity of local and machine chicken egg white and amphotericin B was 12, 11, and 16 mm, respectively.
Conclusion: Local and machine chicken egg whites have antifungal properties against C. albicans.
Materials and Methods: This study was conducted experimentally in May 2023 in the Microbiology Laboratory of Khalkhal Faculty of Medical Sciences. This research tested the antifungal effects of local and machine chicken egg whites and amphotericin B using the micro broth dilution and zone of inhibition method. In this experiment, Candida albicans species were placed in the vicinity of different concentrations of substances, and the minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) of these substances on C. albicans were determined according to the guidelines of the Clinical and Laboratory Standards Institute.
This study has been registered with the ethical code IR. IR.KHALUMS.REC.1401.019 in the Ethics Committee of Khalkhal Faculty of Medical Sciences.
Results: Based on the results of the tests, the MIC of local and machine chicken egg whites on C. albicans was 25%, and their MFC was 50%. However, in these concentrations, no growth was observed in the presence of amphotericin B. Moreover, the MIC and MFC values of 25 µl of amphotericin B with 75 µl of local and machine egg whites were 3.125% and 6.25%, respectively. The zone of inhibition for C. albicans in the vicinity of local and machine chicken egg white and amphotericin B was 12, 11, and 16 mm, respectively.
Conclusion: Local and machine chicken egg whites have antifungal properties against C. albicans.
Keywords: : Local and machine chicken egg white, Candida albicans, amphotericin B, antifungal effect
Type of Study: Research |
Subject:
Traditional medicine
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