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Volume 14, Issue 1 (4-2024)
cmja 2024, 14(1): 13-20 |
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Gholami M, Sadegh M. Investing the anti epileptic effect of Aqueous extract of Artemisia absinthium on seizures induced by pentylenetetrazole in male rats. cmja 2024; 14 (1) :13-20
URL: http://cmja.arakmu.ac.ir/article-1-988-en.html
URL: http://cmja.arakmu.ac.ir/article-1-988-en.html
1- Assistant Professor of Physiology, Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran , ma.gholami@arakmu.ac.ir
2- Associate Professor of Physiology, Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
2- Associate Professor of Physiology, Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Abstract: (638 Views)
Introduction:Epilepsy is one of the most common neurological disorders that affect social, economic, and biological aspects of human life. Thus, this study aimed to investigate the effect of Aqueous extract of Artemisia absinthium on PTZ-induced seizures in male rats.
Methods: 30 male rats have been chosen randomly and divided into 5 groups; including a control group, vehicle group, and three experimental groups. Treated rats received an aqueous extract of Artemisia absinthium via i.p. injection at doses of 50, 100, and 200 mg/kg before injection of pentylenetetrazole (PTZ). Thirty minutes after injection with different doses of the herb or distilled water (vehicle group), pentylenetetrazole (PTZ; 80mg/kg) was injected intraperitoneally. Animals were immediately transferred to a special cage and the seizure behaviors were recorded by a camera for 30 minutes. Then latency time of seizure onset, stage 5 seizure duration, and incidence and rate of mortality were measured in the groups. The data analysis was carried out via one-way ANOVA and Tukey post-hoc tests. Moreover, a difference of less than<0.05 was considered
significant.
Results:Our results showed a significant increase in latency to reach stages 4 (P˂0.01) and 5 (P˂0.001)of tonic-clonic seizure at a dose of 100 mg/kg hyssop extract and decreased stage 5 duration (P˂0.05) and rate of mortality.
Conclusion:Intraperitoneally injection of the aqueous extract of Artemisia absinthium has anticonvulsant effects on PTZ-induced seizure in a dose-response manner. Therefore, complementary research is recommended to identify some effective components of this plant on seizures.
Type of Study: Research |
Subject:
Medicinal Plants
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